Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (726211)

R&D Systems | Catalog # MAB5545

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 726211
View all Cytosolic Sulfotransferase 1E1/SULT1E1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cytosolic Sulfotransferase 1E1/SULT1E1
Asn2-Ile294
Accession # P49888

Specificity

Detects human Cytosolic Sulfotransferase 1E1/SULT1E1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human SULT1A1, 1B1, 2A1, 2B1, or 4A1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (726211)

Detection of Human Cytosolic Sulfotransferase 1E1/SULT1E1 antibody by Western Blot.

Detection of Human Cytosolic Sulfotransferase 1E1/SULT1E1 by Western Blot.

Western blot shows lysates of human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cytosolic Sulfotransferase 1E1/SULT1E1 Monoclonal Antibody (Catalog # MAB5545) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 1E1/SULT1E1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (726211)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human liver tissue

Reviewed Applications

Read 1 review rated 4 using MAB5545 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytosolic Sulfotransferase 1E1/SULT1E1

Cytosolic Sulfotransferases catalyze the sulfation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm. SULT1E1 is widely known as an estrogen sulfotransferase and may control estrogen levels by converting free estradiol to its inactive sulfate conjugate (1). Known substrates of this enzyme also include dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol, 4‑nitrophenol, 1-napthol and resveratrol (2, 3). SULT1E1 activity is found in reproductive organs (4), intestine and liver (3, 5).

References

  1. Gong, H. et al. (2008) Cancer Res. 68:7386.
  2. Falany, C.N. et al. (1995) J. Steroid Biochem. Mol. Biol. 52:529.
  3. Miksits, M. et al. 2005, Xenobiotica 35:1101.
  4. Takase, Y. et al. (2007) The Prostate 67:405.
  5. Schrag, M.L. et al. (2004) Drug Metabolism and Disposition 32:1299.
  6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
  7. MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613.
  8. Wu, Z.L., et al. (2002) Faseb J. 16:539.
    9.  

Alternate Names

EST-1, STE, SULT1E1

Entrez Gene IDs

6783 (Human); 20860 (Mouse); 25355 (Rat)

Gene Symbol

SULT1E1

UniProt

P49888

Additional Cytosolic Sulfotransferase 1E1/SULT1E1 Products

Product Documents for Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (726211)

Certificate of Analysis

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Product Specific Notices for Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (726211)

For research use only

Citations for Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (726211)

Customer Reviews for Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (726211) (1)

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  • Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody
    Name: Hua Jiang
    Application: Western Blot
    Sample Tested: Liver, brain and spinal cord and Mouse brain
    Species: Human and Mouse
    Verified Customer | Posted 08/16/2021
    SULT1E1-Reveiw-2021
    Human Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (726211) MAB5545
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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