Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody

R&D Systems | Catalog # AF5826

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cytosolic Sulfotransferase 4A1/SULT4A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SULT4A1 isoform 1
Ala2-Leu284
Accession # Q9BR01

Specificity

Detects human SULT4A1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SULT1A1, rhSULT2A1, and rhSULT1E1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody

Detection of Human Cytosolic Sulfotransferase 4A1/SULT4A1 antibody by Western Blot.

Detection of Human Cytosolic Sulfotransferase 4A1/SULT4A1 by Western Blot.

Western blot shows lysates of human brain cortex and hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5826) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cytosolic Sulfotransferase 4A1/SULT4A1 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
SULT4A1 antibody in Human Brain

SULT4A1 in Human Brain

SULT4A1 was detected in paraffin-embedded sections of human brain (cortex) using Goat Anti-Human SULT4A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5826) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Immunoprecipitation

25 µg/mL
Sample: Cell lysates spiked with Recombinant Human Cytosolic Sulfotransferase 4A1/SULT4A1, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Human brain cortex and hippocampus tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cytosolic Sulfotransferase 4A1/SULT4A1

SULT4A1 (Cytosolic Sulfotransferase 4A1; also BR-STL1/Brain sulfotransferase-like protein) is a 33-36 kDa member of the sulfotransferase 4 family of enzymes. SULT4A1 message is widely expressed. However, it appears to be translated only in brain. SULT4A1 is very unusual in that it is the only SULT that shows minimal activity on synthetic laboratory substrates. Instead, it acts on catecholamines and T4 in a manner that may not involve sulfonation. Human SULT4A1 is 284 amino acids (aa) in length. A weak PAPS binding site is found between aa 246-254. There are two potential isoforms; one that shows a 13 aa substitution for aa 248-284 and a second that contains a 48 aa substitution for aa 202 - 247. Full-length human SULT4A1 is 98% aa identical to mouse SULT4A1.

Alternate Names

BRSTL1, NST, SULT4A1, SULTX3

Entrez Gene IDs

25830 (Human); 29859 (Mouse)

Gene Symbol

SULT4A1

UniProt

Q9BR01

Additional Cytosolic Sulfotransferase 4A1/SULT4A1 Products

Product Documents for Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody

Certificate of Analysis

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Product Specific Notices for Human Cytosolic Sulfotransferase 4A1/SULT4A1 Antibody

For research use only

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Protocols

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