Human DAP10/HCST Antibody

Catalog # Availability Size / Price Qty
MAB9786-100
MAB9786-SP
Detection of DAP10/HCST in U937 cell line by Flow Cytometry.
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Human DAP10/HCST Antibody Summary

Species Reactivity
Human
Specificity
Detects human DAP10/HCST in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 982129
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing human DAP10/HCST
Accession # Q9UBK5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of DAP10/HCST antibody in U937 cell line antibody by Flow Cytometry. View Larger

Detection of DAP10/HCST in U937 cell line by Flow Cytometry. Human U937 histiocytic lymphoma cell line was stained with Mouse Anti-Human DAP10/HCST Monoclonal Antibody (Catalog # MAB9786, filled histogram) or Mouse IgG2A isotype control antibody (Catalog # MAB003, open histogram) followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DAP10/HCST

This gene encodes a transmembrane signaling adaptor that contains a YxxM motif in its cytoplasmic domain. The encoded protein may form part of the immune recognition receptor complex with the C-type lectin-like receptor NKG2D. As part of this receptor complex, this protein may activate phosphatidylinositol 3-kinase dependent signaling pathways through its intracytoplasmic YxxM motif. This receptor complex may have a role in cell survival and proliferation by activation of NK and T cell responses. Alternative splicing results in two transcript variants encoding different isoforms.

Entrez Gene IDs
10870 (Human); 23900 (Mouse); 474146 (Rat)
Alternate Names
DAP10; DAP10PIK3AP; DKFZP586C1522; HCST; hematopoietic cell signal transducer; KAP10; KAP10DNAX-activation protein 10; kinase assoc pro of ~10kDa; Kinase Assoc Protein; Membrane protein DAP10; phosphoinositide-3-kinase adaptor protein; PIK3AP; Transmembrane adapter protein KAP10

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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