Human DC-SCRIPT/ZNF366 Antibody

Catalog # Availability Size / Price Qty
AF4707
AF4707-SP

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Detection of DC‑SCRIPT/ZNF366 in Human Mature Dendritic Cells by Flow Cytometry.
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Citations (4)
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Human DC-SCRIPT/ZNF366 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DC-SCRIPT/ZNF366 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) ZNF24 and rhZNF206 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human DC-SCRIPT/ZNF366
Met559-Ile744
Accession # Q8N895
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human DC-SCRIPT/ZNF366
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of DC‑SCRIPT/ZNF366 antibody in Human Mature Dendritic Cells antibody by Flow Cytometry. View Larger

Detection of DC‑SCRIPT/ZNF366 in Human Mature Dendritic Cells by Flow Cytometry. Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+cells treated with 20 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 7 days and 1 µg/mL LPS, 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA), and 20 ng/mL Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) for last 24 hours were stained with Human DC-SCRIPT/ZNF366 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4707, filled histogram), or control antibody (Catalog # AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Phycoerythrin (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DC-SCRIPT/ZNF366

DC-SCRIPT (dendritic cell-specific transcript; also ZNF366) is an 85 kDa member of the Cys-Cys:His-His subfamily of zinc-finger proteins. This subfamily is principally involved in protein-DNA interactions. DC-SCRIPT appears to be DC specific, and is expressed in myeloid DC, plasmacytoid DC and Langerhans cells. Human DC-SCRIPT is 744 amino acids (aa) in length. It contains an R-K-R-K NLS (aa 77-80), a Pro-rich region (aa 110-219), eleven C2H2-type zinc-fingers (aa 255‑556) and an extended acidic region (aa 586-698). Over aa 559-744, human DC-SCRIPT is 68% aa identical to mouse DC-SCRIPT.

Long Name
Dendritic Cell-specific Transcript
Entrez Gene IDs
167465 (Human); 238803 (Mouse)
Alternate Names
DCSCRIPT; DC-SCRIPT; FLJ39796; zinc finger protein 366; ZNF366

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Citations for Human DC-SCRIPT/ZNF366 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Monocytes differentiate along two alternative pathways during sterile inflammation
    Authors: Javiera Villar, Léa Ouaknin, Adeline Cros, Elodie Segura
    EMBO reports
  2. DC-SCRIPT regulates glucocorticoid receptor function and expression of its target GILZ in dendritic cells.
    Authors: Hontelez, Saartje, Karthaus, Nina, Looman, Maaike W, Ansems, Marleen, Adema, Gosse J
    J Immunol, 2013-02-25;190(7):3172-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Dendritic cell-specific transcript: dendritic cell marker and regulator of TLR-induced cytokine production.
    Authors: Hontelez S, Ansems M, Karthaus N
    J. Immunol., 2012-05-21;189(1):138-45.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr, Western Blot
  4. DC-SCRIPT: nuclear receptor modulation and prognostic significance in primary breast cancer.
    Authors: Ansems M, Hontelez S, Looman MW, Karthaus N, Bult P, Bonenkamp JJ, Jansen JH, Sweep FC, Span PN, Adema GJ
    J. Natl. Cancer Inst., 2009-12-14;102(1):54-68.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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