Human DEC-205/CD205 Antibody

Catalog # Availability Size / Price Qty
Detection of Human DEC‑205/CD205 by Western Blot.
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Human DEC-205/CD205 Antibody Summary

Species Reactivity
Detects human DEC‑205/CD205 in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human DEC‑205/CD205
Accession # O60449
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human DEC-205/CD205 antibody by Western Blot. View Larger

Detection of Human DEC‑205/CD205 by Western Blot. Western blot shows lysates of human thymus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DEC-205/CD205 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2047) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DEC-205/ CD205 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry DEC-205/CD205 antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

DEC‑205/CD205 in Human PBMCs. DEC-205/CD205 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human DEC-205/CD205 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2047) for 3 hours at room temperature. Cells were stained with the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DEC-205/CD205

DEC-205, also known as CD205 and lymphocyte antigen 75 (Ly 75), is a type I transmembrane protein that is primarily expressed on dendritic cells and thymic epithelial cells. The extracellular region of DEC-205 contains ten C-type lectin-like domains, a fibronectin type II domain and a ricin B-type lectin domain. DEC-205 functions as an endocytic receptor for antigens. The recombinant protein used to generate the anti‑human DEC-205 antibody contains the first two C-type lectin domains.

Entrez Gene IDs
4065 (Human); 17076 (Mouse)
Alternate Names
CD205; DEC205; DEC-205; Ly75

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


Reviews for Human DEC-205/CD205 Antibody

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Human DEC-205/CD205 Antibody
By Rambabu Surabattula on 02/13/2023
Application: ELISA Sample Tested: Serum and Plasma Species: Human

workign good with 1:5 dilution for the detection of human DEC 205 in the serum or plasma samples.