Detects human Desmoglein-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25‑50% cross‑reactivity with recombinant human (rh) Desmoglein-1 is observed and no cross-reactivity with rhDesmoglein-3 is observed.
Monoclonal Mouse IgG1 Clone # 141409
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human Desmoglein-2 Ala50-Gly608 (predicted) Accession # CAA81226
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human Desmoglein‑2 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Desmoglein‑2 Monoclonal Antibody (Catalog # MAB947) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Desmoglein‑2 at approximately 90-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Desmoglein‑2 in NHEK Human Cells.
Desmoglein‑2 was detected in immersion fixed NHEK human normal epidermal keratinocytes using Mouse Anti-Human Desmoglein‑2 Monoclonal Antibody (Catalog # MAB947) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell junctions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Desmoglein‑2 by Simple WesternTM.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Desmoglein‑2 at approximately 120 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Desmoglein‑2 Monoclonal Antibody (Catalog # MAB947). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Desmoglein-2 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. Human Desmoglein-2 is a type I transmembrane glycoprotein of 1117 amino acid (aa) residues with a 23 aa signal peptide and a 25 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, desmogleins have R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tails of desmogleins interact with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the desmogleins with the intermediate filaments. Desmoglein-2 has been shown to be important in establishing cell-cell adhesion and function in epithelial cells. Desmoglein-2 was originally identified in colon carcinoma and colon, and was named HDGC (human desmoglein colon).
Nollet, R. et al. (2000) J. Mol. Biol. 299:551.
Elias, P. et al. (2001) J. Cell Biol. 153:243.
Arnemann, J. et al. (1992) Genomics 13:484.
Entrez Gene IDs:
1829 (Human); 13511 (Mouse)
ARVC10; ARVD10; Cadherin family member 5; CDHF5; CDHF5MGC117034; CMD1BB; desmoglein 2; Desmoglein2; Desmoglein-2; DSG2; HDGC; MGC117036; MGC117037
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