Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 141409
View all Desmoglein-2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Desmoglein-2
Ala50-Gly608 (predicted)
Accession # CAA81226

Specificity

Detects human Desmoglein-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25‑50% cross‑reactivity with recombinant human (rh) Desmoglein-1 is observed and no cross-reactivity with rhDesmoglein-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Desmoglein‑2 Antibody

Detection of Human Desmoglein-2 antibody by Western Blot.

Detection of Human Desmoglein‑2 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Desmoglein-2 Monoclonal Antibody (Catalog # MAB947) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Desmoglein-2 at approximately 90-160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Desmoglein-2 antibody in NHEK Human Cells by Immunocytochemistry (ICC).

Desmoglein‑2 in NHEK Human Cells.

Desmoglein-2 was detected in immersion fixed NHEK human normal epidermal keratinocytes using Mouse Anti-Human Desmoglein-2 Monoclonal Antibody (Catalog # MAB947) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell junctions. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Desmoglein-2 antibody in A431 Human Cell Line by Immunocytochemistry (ICC).

Desmoglein‑2 in A431 Human Cell Line.

Desmoglein-2 was detected in immersion fixed A431 human epithelial carcinoma cell line wildtype and knockout using Mouse Anti-Human Desmoglein-2 Monoclonal Antibody (Catalog # MAB947) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Desmoglein‑2 antibody by Simple WesternTM.

Detection of Human Desmoglein‑2 by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Desmoglein‑2 at approximately 120 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Desmoglein‑2 Monoclonal Antibody (Catalog # MAB947). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Desmoglein-2 by Immunohistochemistry

Detection of Desmoglein-2 by Immunohistochemistry

DSG2 promotes the adhesion of MM plasma cells to BM endothelial cells and is co‐regulated with N‐cadherin. (A) BM trephine biopsies from three MM patients were stained for DSG2 by immunohistochemistry; a representative example shows the isotype control (iso ctl) and DSG2‐stained section with a DSG2‐expressing blood vessel (black arrow) and MM PC (red arrows). (B) Expression of DSG2 by the TrHBMEC cell line was assessed by flow cytometry in the parent culture (left); after sorting on DSG2 expression to enrich for BMECDSG2 cells (centre); or after extended passage of the BMECDSG2 cells (right). (C) Adhesion of KMS‐11 cells (± shDSG2) to a monolayer of BMECDSG2 cells for 15 min followed by extensive washing and cell adhesion quantified by imaging the GFP reporter in the KMS‐11 cells. Shown in (C, left image) are representative fluorescent images (scale bar = 100 μm) while (C, right image) shows a summary graph of four independent experiments (Mann–Whitney test *P < 0.05 compared to shNT control). (D) Adhesion of RPMI8226 cells (± siDSG2) to a monolayer of BMECDSG2 cells as above. Representative fluorescent images (scale bar = 100 μm) are shown in (D, left image) while (D, right image) shows a summary graph of three independent experiments (Mann–Whitney test *P < 0.05 compared to siNT control). (E) Gene expression values for DSG2 and CDH2 (N‐cadherin) were extracted from dataset GSE4581. Samples in the MS subgroup are shown in red while others (MS‐negative) are shown in black. Quadrants were set visually to highlight the four distinct subsets defined by individual or co‐expression of DSG2 and CDH2. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34245117), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Desmoglein‑2 Antibody

Application
Recommended Usage

Immunocytochemistry

5-25 µg/mL
Sample: Immersion fixed NHEK human normal epidermal keratinocytes and A431 human epithelial carcinoma cell line

Simple Western

20 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line under reducing conditions only

Reviewed Applications

Read 2 reviews rated 5 using MAB947 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Desmoglein-2

Desmoglein-2 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. Human Desmoglein-2 is a type I transmembrane glycoprotein of 1117 amino acid (aa) residues with a 23 aa signal peptide and a 25 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, desmogleins have R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tails of desmogleins interact with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the desmogleins with the intermediate filaments. Desmoglein-2 has been shown to be important in establishing cell-cell adhesion and function in epithelial cells. Desmoglein-2 was originally identified in colon carcinoma and colon, and was named HDGC (human desmoglein colon).

References

  1. Nollet, R. et al. (2000) J. Mol. Biol. 299:551.
  2. Elias, P. et al. (2001) J. Cell Biol. 153:243.
  3. Arnemann, J. et al. (1992) Genomics 13:484.

Alternate Names

CDHF5, Desmoglein2, DSG2, HDGC

Entrez Gene IDs

1829 (Human); 13511 (Mouse)

Gene Symbol

DSG2

UniProt

CAA81226

Additional Desmoglein-2 Products

Product Documents for Human Desmoglein‑2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Desmoglein‑2 Antibody

For research use only

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

Citations for Human Desmoglein‑2 Antibody

Customer Reviews for Human Desmoglein‑2 Antibody (2)

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  • Human Desmoglein-2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: A431 human epithelial carcinoma cell line
    Species: Human
    Verified Customer | Posted 12/29/2021
    Human Desmoglein‑2 Antibody MAB947
  • Human Desmoglein-2 Antibody
    Name: Preethi Vijayaraj
    Application: Immunohistochemistry
    Sample Tested: Adult lung
    Species: Human
    Verified Customer | Posted 02/15/2019
    Human Desmoglein‑2 Antibody MAB947

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