Detection of Human DIAPH-2 by Western Blot. Western blot shows lysates of human testis tissue and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human DIAPH-2 Monoclonal Antibody (Catalog # MAB6806) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DIAPH-2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
DIAPH2 (diaphenous homolog 2), also called DRF2 (diaphanous-related formin 2), is an 1101 amino acid (aa), 126 kDa (calculated) cytoplasmic protein in the Diaphenous family of actin binding proteins. Disruption of DIAPH2 has been correlated with premature ovarian failure in humans. Within the region used as an immunogen, human DIAPH2 shares 89% and 90% aa identity with mouse and rat DIAPH2, respectively. Two additional isoforms have been described. One, called hDia2C (1097 aa), is lacking aa 45-55, but has 7 aa inserted after aa 149. This form specifically binds RhoD and restricts movement of early endosomes along actin filaments. A 1106 aa form diverges after aa 1080.
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