Human DIAPH-2 Antibody Summary
Accession # O60879
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human DIAPH-2 by Western Blot. Western blot shows lysates of human testis tissue and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human DIAPH-2 Monoclonal Antibody (Catalog # MAB6806) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DIAPH-2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
DIAPH2 (diaphenous homolog 2), also called DRF2 (diaphanous-related formin 2), is an 1101 amino acid (aa), 126 kDa (calculated) cytoplasmic protein in the Diaphenous family of actin binding proteins. Disruption of DIAPH2 has been correlated with premature ovarian failure in humans. Within the region used as an immunogen, human DIAPH2 shares 89% and 90% aa identity with mouse and rat DIAPH2, respectively. Two additional isoforms have been described. One, called hDia2C (1097 aa), is lacking aa 45-55, but has 7 aa inserted after aa 149. This form specifically binds RhoD and restricts movement of early endosomes along actin filaments. A 1106 aa form diverges after aa 1080.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human DIAPH-2 Antibody
There are currently no reviews for this product. Be the first to review Human DIAPH-2 Antibody and earn rewards!
Have you used Human DIAPH-2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image