Human DLL3 Antibody

Catalog # Availability Size / Price Qty
MAB4315
MAB4315-SP
Product Details
FAQs
Supplemental Products
Reviews (8)

Human DLL3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DLL3 in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) DLL1 or rhDLL4.
Source
Monoclonal Mouse IgG2B Clone # 378703
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human DLL3
Ala27-Pro488
Accession # Q9NYJ7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Complete Your Experiment

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human DLL3

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL3

Delta-like 3 (DLL3) is a transmembrane Delta-like protein that contains one DSL domain and six EGF-like repeats. DLL3 is critical for somite segmentation and neurogenesis during early embryonic development. It does not bind Notch receptors but functions as an antagonist of Notch signaling. Defects in DLL3 cause axial skeletal abnormalities in spondylocostal dysostosis. Within the extracellular domain, human DLL3 shares 84% amino acid sequence identity with mouse and rat DLL3.

Long Name
Delta-like 3
Entrez Gene IDs
10683 (Human); 13389 (Mouse); 114125 (Rat)
Alternate Names
delta (Drosophila)-like 3; Delta3; delta-like 3 (Drosophila); delta-like protein 3; DLL3; Drosophila Delta homolog 3; Pudgy; SCDO1; SCDO1delta3

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human DLL3 Antibody

Average Rating: 4.3 (Based on 8 Reviews)

5 Star
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4 Star
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Human DLL3 Antibody
By Anonymous on 12/08/2021
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human

Human DLL3 Antibody
By Anonymous on 12/23/2020
Application: Flow Sample Tested: NCI-H82 lung cancer cell line Species: Human

Detection of DLL3 on lung cancer cell line NCI-H82. NCI-H82 cells were treated with 100 nM of the human DLL3 antibody (catalog # MAB4315) or mouse IgG2b isotype control, followed by a secondary antibody goat a-mouse IgG Fc APC. Isotype control (RED), human DLL3 antibody (BLUE).


Human DLL3 Antibody
By Anonymous on 01/03/2020
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human DLL3 Antibody
By Anonymous on 02/21/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

By Jignesh Ganeshbhai Patel on 09/10/2018
Application: WB Sample Tested: Blood mononuclear cells (PBMCs) Species: Human

By Jignesh Patel on 09/10/2018
Application: ICC/IF Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human

By Jignesh Patel on 08/21/2018
Application: WB Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human

Human DLL3 Antibody
By Anonymous on 04/07/2016
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human

Recombinant DLL3 ECD Fc fusion was expressed transiently in 293. Generated conditioned media was run over Protein A column, then desalted, then polished on SEC column. The picture shown is a Western Blot for the collected SEC fractions, using MAB4315 as the primary antibody, followed by an anti-murine secondary antibody.