Detection of Human DOK3 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 0.25 µg/mL of Mouse Anti-Human DOK3 Monoclonal Antibody (Catalog # MAB4795) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DOK3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
DOK3 (downstream of kinase 3) is a 53 kDa (predicted) member of the DOK family of cytoplasmic adaptor proteins and is expressed in B cells and macrophages. Human DOK3 cDNA encodes 496 amino acids (aa) including one pleckstrin homology region (aa 63‑179) and an IRS-type phosphotyrosine binding domain (PTB) (aa 213‑317). Alternate splicing generates 330, 228 and 216 aa isoforms with N-terminal and C-terminal truncations and substitutions. Human DOK3 shares approximately 80% aa sequence identity with mouse and rat DOK3 within aa 181‑271, which are common to all four isoforms. Phosphorylation of tyrosine residues in DOK3 regulates its interaction with SH2 and SH3 containing proteins, leading to inhibition of signaling from immunoreceptors.
Downstream of Tyrosine Kinase 3
Entrez Gene IDs:
79930 (Human); 27261 (Mouse); 306760 (Rat)
docking protein 3; DOK3; DOKL; Dok-like protein; Downstream of tyrosine kinase 3; FLJ22570; FLJ39939
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