Dopamine Receptor D1 (DRD1) is a 50 kDa member of class 1 GPCR superfamily and is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. DRD1 is associated with nicotine dependence, schizophrenia, and systolic blood pressure levels.
Human Dopamine D1R/DRD1 Antibody
R&D Systems | Catalog # MAB8276
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Saimiri sciureus (Common Squirrel Monkey)
Applications
Validated:
Immunohistochemistry, Flow Cytometry, CyTOF-ready
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 887438
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Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human Dopamine D1 R/DRD1
Met1-Thr446
Accession # P21728
Met1-Thr446
Accession # P21728
Specificity
Stains human Dopamine D1 R/DRD1 transfected cells but not irrelevant transfectants in flow cytometry.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Dopamine D1R/DRD1 Antibody
Detection of Dopamine D1 R/DRD1 in HEK293 Human Cell Line Transfected with Human Dopamine D1/DRD1 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with human Dopamine D1/DRD1 and eGFP was stained with either (A) Mouse Anti-Human Dopamine D1 R/DRD1 Monoclonal Antibody (Catalog # MAB8276) or (B) Mouse IgG2AIsotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Dopamine D1 R/DRD1 in Human Brain.
Dopamine D1 R/DRD1 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human Dopamine D1 R/DRD1 Monoclonal Antibody (Catalog # MAB8276) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human Dopamine D1R/DRD1 by Immunocytochemistry/ Immunofluorescence
Expression of MCT8 and OATP1C1 in D1-MSN (direct pathway medium-sized spiny neurons) in human and macaque striatum. Representative confocal microscope compositions from double-stained sections for MCT8 (green) or OATP1C1 (green) and for the D1-MSN marker DRD1 (red) in caudate nucleus or putamen. Merged images (right side) show the colocalization of both signals. Coexpression of MCT8 and DRD1 is observed in human (A–C) and macaque (D–F) striatum. Coexpression of OATP1C1 and DRD1 is observed in human (G–I) and macaque (J–L) striatum. Counterstaining with DAPI (blue) shows nuclei of all cells. Note that in humans the MCT8 signal is located mainly at the cell membrane, while in macaques it is located at the membrane and in the cytoplasm. Cd: caudate nucleus; DRD1: Dopamine receptor type 1; D1-MSN: D1 receptor-expressing medium-sized spiny neurons; Put: putamen. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37298594), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Dopamine D1R/DRD1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human Dopamine D1/DRD1 and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with human Dopamine D1/DRD1 and eGFP
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate nucleus)
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate nucleus)
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Dopamine D1R/DRD1
Long Name
Dopamine D1 Receptor
Alternate Names
DADR, Dopamine D1 R, DRD1
Gene Symbol
DRD1
UniProt
Additional Dopamine D1R/DRD1 Products
Product Documents for Human Dopamine D1R/DRD1 Antibody
Product Specific Notices for Human Dopamine D1R/DRD1 Antibody
For research use only
Related Research Areas
Citations for Human Dopamine D1R/DRD1 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars