Human DPPIV/CD26 Antibody
Human DPPIV/CD26 Antibody Summary
Asp34-Pro766
Accession # Q53TN1
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human DPPIV/CD26 by Western Blot. Western blot shows lysate of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human DPPIV/CD26 by Western Blot. Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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DPPIV/CD26 in Human PBMCs. DPPIV/CD26 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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DPPIV/CD26 in Human Psoriatic Skin. DPPIV/CD26 was detected in immersion fixed paraffin-embedded sections of human psoriatic skin using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of Human DPPIV/CD26 by Simple WesternTM. Simple Western lane view shows lysates of LoVo human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 168 kDa (as indicated) using 2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DPPIV/CD26
DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides. It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain. The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.
DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a cofactor for entry of HIV in CD4+ cells. It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans. It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine. It degrades peptide hormones such as glucagon. It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis.
Product Datasheets
Citations for Human DPPIV/CD26 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Seminal-Plasma-Mediated Effects on Sperm Performance in Humans
Authors: T Turunen, M Magris, M Malinen, J Kekäläinen
Cells, 2022;11(14):.
Species: Human
Sample Types: Seminal Plasma
Applications: Western Blot -
Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma
Authors: C Nadolny, X Zhang, Q Chen, SF Hashmi, W Ali, C Hemme, N Ahsan, Y Chen, R Deng
American journal of cancer research, 2021;11(10):4746-4767.
Species: Human
Sample Types: Cell Lysate
Applications: Western Blot -
Functional inhibition of cancer stemness-related protein DPP4 rescues tyrosine kinase inhibitor resistance in renal cell carcinoma
Authors: S Kamada, T Namekawa, K Ikeda, T Suzuki, M Kagawa, H Takeshita, A Yano, K Okamoto, T Ichikawa, K Horie-Inou, S Kawakami, S Inoue
Oncogene, 2021;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Stability of human salivary extracellular vesicles containing dipeptidyl peptidase IV under simulated gastrointestinal tract conditions
Authors: Y Ogawa, Y Akimoto, M Ikemoto, Y Goto, A Ishikawa, S Ohta, Y Takase, H Kawakami, M Tsujimoto, R Yanoshita
Biochemistry and Biophysics Reports, 2021;27(0):101034.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Diagnostic significance of apical membranous and cytoplasmic dot-like CD26 expression in encapsulated follicular variant of papillary thyroid carcinoma: a useful marker for capsular invasion
Authors: S Takagi, M Hirokawa, K Nagashima, M Higuchi, K Kadota, R Ishikawa, M Sato, A Miyauchi, Y Miyake, R Haba
Endocr. J., 2020;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Chromatin and transcriptome changes in human myoblasts show spatio-temporal correlations and demonstrate DPP4 inhibition in differentiated myotubes
Authors: TJ Kolanowski, N Rozwadowsk, A Zimna, M Nowaczyk, M Siatkowski, W ?ab?d?, E Wiland, J Gapi?ski, S Jurga, M Kurpisz
Sci Rep, 2020;10(1):14336.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Chromatin and transcriptome changes in human myoblasts show spatio-temporal correlations and demonstrate DPP4 inhibition in differentiated myotubes
Authors: TJ Kolanowski, N Rozwadowsk, A Zimna, M Nowaczyk, M Siatkowski, W ?ab?d?, E Wiland, J Gapi?ski, S Jurga, M Kurpisz
Sci Rep, 2020;10(1):14336.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Teneligliptin prevents doxorubicin-induced inflammation and apoptosis in H9c2 cells
Authors: W Peng, D Rao, M Zhang, Y Shi, J Wu, G Nie, Q Xia
Arch. Biochem. Biophys., 2019;0(0):108238.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Entry of Scotophilus Bat Coronavirus-512 and Severe Acute Respiratory Syndrome Coronavirus in Human and Multiple Animal Cells
Authors: YN Chen, HC Hsu, SW Wang, HC Lien, HT Lu, SK Peng
Pathogens, 2019;8(4):.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Insights into replicative senescence of human testicular peritubular cells
Authors: N Schmid, F Flenkentha, JB Stöckl, KG Dietrich, FM Köhn, JU Schwarzer, L Kunz, M Luckner, G Wanner, GJ Arnold, T Fröhlich, A Mayerhofer
Sci Rep, 2019;9(1):15052.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Development of novel monoclonal antibodies with specific binding affinity for denatured human CD26 in formalin-fixed paraffin-embedded and decalcified specimens
Authors: R Hatano, T Yamada, H Madokoro, H Otsuka, E Komiya, T Itoh, Y Narita, S Iwata, H Yamazaki, S Matsuoka, NH Dang, K Ohnuma, C Morimoto
PLoS ONE, 2019;14(6):e0218330.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Experimental infection of dromedaries with Middle East respiratory syndrome-Coronavirus is accompanied by massive ciliary loss and depletion of the cell surface receptor dipeptidyl peptidase 4
Authors: AK Haverkamp, A Lehmbecker, I Spitzbarth, W Widagdo, BL Haagmans, J Segalés, J Vergara-Al, A Bensaid, JMA van den Br, ADME Osterhaus, W Baumgärtne
Sci Rep, 2018;8(1):9778.
Species: Camelid
Sample Types: Whole Cells
Applications: ICC -
Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells
Authors: H Chu, CM Chan, X Zhang, Y Wang, S Yuan, J Zhou, RK Au-Yeung, KH Sze, D Yang, H Shuai, Y Hou, C Li, X Zhao, VK Poon, SP Leung, ML Yeung, J Yan, G Lu, DY Jin, GF Gao, JF Chan, KY Yuen
J. Biol. Chem., 2018;0(0):.
Applications: Western Blot -
Discovery of novel bat coronaviruses in south China that use the same receptor as MERS coronavirus
Authors: CM Luo, N Wang, XL Yang, HZ Liu, W Zhang, B Li, B Hu, C Peng, QB Geng, GJ Zhu, F Li, ZL Shi
J. Virol., 2018;0(0):.
Species: Bat
Sample Types: Whole Cells
Applications: Neutralization -
A spike-modified Middle East respiratory syndrome coronavirus (MERS-CoV) infectious clone elicits mild respiratory disease in infected rhesus macaques
Authors: AS Cockrell, JC Johnson, IN Moore, DX Liu, KW Bock, MG Douglas, RL Graham, J Solomon, L Torzewski, C Bartos, R Hart, RS Baric, RF Johnson
Sci Rep, 2018;8(1):10727.
Species: Primate
Sample Types: Whole Tissue
Applications: IHC -
Identification of sialic acid-binding function for the Middle East respiratory syndrome coronavirus spike glycoprotein
Authors: W Li, RJG Hulswit, I Widjaja, VS Raj, R McBride, W Peng, W Widagdo, MA Tortorici, B van Dieren, Y Lang, JWM van Lent, JC Paulson, CAM de Haan, RJ de Groot, FJM van Kuppev, BL Haagmans, BJ Bosch
Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
First-in-human phase 1 of YS110, a monoclonal antibody directed against CD26 in advanced CD26-expressing cancers
Authors: E Angevin, N Isambert, V Trillet-Le, B You, J Alexandre, G Zalcman, P Vielh, F Farace, F Valleix, T Podoll, Y Kuramochi, I Miyashita, O Hosono, NH Dang, K Ohnuma, T Yamada, Y Kaneko, C Morimoto
Br. J. Cancer, 2017;116(9):1126-1134.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Elevated CD26 Expression by Skin Fibroblasts Distinguishes a Profibrotic Phenotype Involved in Scar Formation Compared to Gingival Fibroblasts
Authors: W Mah, G Jiang, D Olver, C Gallant-Be, C Wiebe, DA Hart, L Koivisto, H Larjava, L Häkkinen
Am. J. Pathol., 2017;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Glycosylation of mouse DPP4 plays a role in inhibiting Middle East respiratory syndrome coronavirus infection.
Authors: Peck K, Cockrell A, Yount B, Scobey T, Baric R, Heise M
J Virol, 2015;89(8):4696-9.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Regulation of pulmonary graft-versus-host disease by IL-26+CD26+CD4 T lymphocytes.
Authors: Ohnuma K, Hatano R, Aune T, Otsuka H, Iwata S, Dang N, Yamada T, Morimoto C
J Immunol, 2015;194(8):3697-712.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Characterization of dedifferentiating human mature adipocytes from the visceral and subcutaneous fat compartments: fibroblast-activation protein alpha and dipeptidyl peptidase 4 as major components of matrix remodeling.
Authors: Lessard J, Pelletier M, Biertho L, Biron S, Marceau S, Hould F, Lebel S, Moustarah F, Lescelleur O, Marceau P, Tchernof A
PLoS ONE, 2015;10(3):e0122065.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
High secretion of interferons by human plasmacytoid dendritic cells upon recognition of Middle East respiratory syndrome coronavirus.
Authors: Scheuplein V, Seifried J, Malczyk A, Miller L, Hocker L, Vergara-Alert J, Dolnik O, Zielecki F, Becker B, Spreitzer I, Konig R, Becker S, Waibler Z, Muhlebach M
J Virol, 2015;89(7):3859-69.
Species: Human
Sample Types: Whole Cells
Applications: Blocking -
Generation of a transgenic mouse model of Middle East respiratory syndrome coronavirus infection and disease.
Authors: Agrawal A, Garron T, Tao X, Peng B, Wakamiya M, Chan T, Couch R, Tseng C
J Virol, 2015;89(7):3659-70.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Adenosine deaminase acts as a natural antagonist for dipeptidyl peptidase 4-mediated entry of the Middle East respiratory syndrome coronavirus.
Authors: Raj, V Stalin, Smits, Saskia L, Provacia, Lisette, van den Brand, Judith M, Wiersma, Lidewij, Ouwendijk, Werner J, Bestebroer, Theo M, Spronken, Monique, van Amerongen, Geert, Rottier, Peter J, Fouchier, Ron A M, Bosch, Berend J, Osterhaus, Albert D, Haagmans, Bart L
J Virol, 2014;88(3):1834-8.
Species: Ferret
Sample Types: Whole Cells
Applications: Flow Cytometry -
Lineage-specific regulation of epigenetic modifier genes in human liver and brain.
Authors: Weng, Matthias, Natarajan, Karthick, Scholz, Diana, Ivanova, Violeta, Sachinidis, Agapios, Hengstler, Jan G, Waldmann, Tanja, Leist, Marcel
PLoS ONE, 2014;9(7):e102035.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Regulation of somatostatin receptor 4-mediated cytostatic effects by CD26 in malignant pleural mesothelioma.
Authors: Yamamoto, J, Ohnuma, K, Hatano, R, Okamoto, T, Komiya, E, Yamazaki, H, Iwata, S, Dang, N H, Aoe, K, Kishimoto, T, Yamada, T, Morimoto, C
Br J Cancer, 2014;110(9):2232-45.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation -
CD26 expression on T-anaplastic large cell lymphoma (ALCL) line Karpas 299 is associated with increased expression of versican and MT1-MMP and enhanced adhesion.
Authors: Havre, Pamela A, Dang, Long H, Ohnuma, Kei, Iwata, Satoshi, Morimoto, Chikao, Dang, Nam H
BMC Cancer, 2013;13(0):517.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection.
Authors: Eckerle I, Muller M, Kallies S, Gotthardt D, Drosten C
Virol J, 2013;10(0):359.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Lymphatic-specific expression of dipeptidyl peptidase IV and its dual role in lymphatic endothelial function.
Authors: Shin JW, Jurisic G, Detmar M
Exp. Cell Res., 2008;314(16):3048-56.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Distinct gene expression-defined classes of gastrointestinal stromal tumor.
Authors: Yamaguchi U, Nakayama R, Honda K, Ichikawa H, Hasegawa T, Shitashige M, Ono M, Shoji A, Sakuma T, Kuwabara H, Shimada Y, Sasako M, Shimoda T, Kawai A, Hirohashi S, Yamada T
J. Clin. Oncol., 2008;26(25):4100-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
Trypsin Treatment Unlocks Barrier for Zoonotic Bat Coronavirus Infection.
Authors: Menachery V, Dinnon K, Yount B, McAnarney E, Gralinski L, Hale A, Graham R, Scobey T, Anthony S, Wang L, Graham B, Randell S, Lipkin W, Baric R
J Virol, 0;94(5):.
Species: Human
Sample Types: cell culture
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CD26/DPP4 cell-surface expression in bat cells correlates with bat cell susceptibility to Middle East respiratory syndrome coronavirus (MERS-CoV) infection and evolution of persistent infection.
Authors: Cai Y, Yu S, Postnikova E, Mazur S, Bernbaum J, Burk R, Zhang T, Radoshitzky S, Muller M, Jordan I, Bollinger L, Hensley L, Jahrling P, Kuhn J
PLoS ONE, 0;9(11):e112060.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
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Serum-starved HepG2 cells were treated with 100 ng/ml phosphoglucose isomerase (PGI) in minimal essential medium for 2 days, following which cells were harvested and analyzed by Western blotting using the CD26 antibody (1000 fold dilution). The study shows that PGI induces the expression of CD26.
IHC-paraffin
working dilution: 1ug/mL
with Bond Refine polymer Detection
rabbit anti-goat IgG as a Linker antibody
epitope retrieval: basic (BOND ER2)
