Human DPPIV/CD26 Antibody

Catalog # Availability Size / Price Qty
AF1180
AF1180-SP
Human DPPIV/CD26 Antibody in Western Blot
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Product Details
Citations (23)
FAQs
Supplemental Products
Reviews (2)

Human DPPIV/CD26 Antibody Summary

Species Reactivity
Human
Specificity
Detects human DPPIV/CD26 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse DPPIV is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human DPPIV/CD26 (Catalog # 1180-SE)
Asp34-Pro766
Accession # Q53TN1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below
Immunocytochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human DPPIV/CD26 by Western Blot. View Larger

Detection of Human DPPIV/CD26 by Western Blot. Western blot shows lysate of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human DPPIV/CD26 by Western Blot. View Larger

Detection of Human DPPIV/CD26 by Western Blot. Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry DPPIV/CD26 in Human PBMCs. View Larger

DPPIV/CD26 in Human PBMCs. DPPIV/CD26 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry DPPIV/CD26 in Human Psoriatic Skin. View Larger

DPPIV/CD26 in Human Psoriatic Skin. DPPIV/CD26 was detected in immersion fixed paraffin-embedded sections of human psoriatic skin using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human DPPIV/CD26 by Simple Western<sup>TM</sup>. View Larger

Detection of Human DPPIV/CD26 by Simple WesternTM. Simple Western lane view shows lysates of LoVo human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 168 kDa (as indicated) using 2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPIV/CD26

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides. It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain. The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.

DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a cofactor for entry of HIV in CD4+ cells. It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans. It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine. It degrades peptide hormones such as glucagon. It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis.

Long Name
Dipeptidyl-peptidase IV
Entrez Gene IDs
1803 (Human); 13482 (Mouse); 102133935 (Cynomolgus Monkey)
Alternate Names
ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5

Product Datasheets

Citations for Human DPPIV/CD26 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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  1. Insights into replicative senescence of human testicular peritubular cells
    Authors: N Schmid, F Flenkentha, JB Stöckl, KG Dietrich, FM Köhn, JU Schwarzer, L Kunz, M Luckner, G Wanner, GJ Arnold, T Fröhlich, A Mayerhofer
    Sci Rep, 2019;9(1):15052.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Teneligliptin prevents doxorubicin-induced inflammation and apoptosis in H9c2 cells
    Authors: W Peng, D Rao, M Zhang, Y Shi, J Wu, G Nie, Q Xia
    Arch. Biochem. Biophys., 2019;0(0):108238.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Entry of Scotophilus Bat Coronavirus-512 and Severe Acute Respiratory Syndrome Coronavirus in Human and Multiple Animal Cells
    Authors: YN Chen, HC Hsu, SW Wang, HC Lien, HT Lu, SK Peng
    Pathogens, 2019;8(4):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. A spike-modified Middle East respiratory syndrome coronavirus (MERS-CoV) infectious clone elicits mild respiratory disease in infected rhesus macaques
    Authors: AS Cockrell, JC Johnson, IN Moore, DX Liu, KW Bock, MG Douglas, RL Graham, J Solomon, L Torzewski, C Bartos, R Hart, RS Baric, RF Johnson
    Sci Rep, 2018;8(1):10727.
    Species: Primate
    Sample Types: Whole Tissue
    Applications: IHC
  5. Discovery of novel bat coronaviruses in south China that use the same receptor as MERS coronavirus
    Authors: CM Luo, N Wang, XL Yang, HZ Liu, W Zhang, B Li, B Hu, C Peng, QB Geng, GJ Zhu, F Li, ZL Shi
    J. Virol., 2018;0(0):.
    Species: Bat
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells
    Authors: H Chu, CM Chan, X Zhang, Y Wang, S Yuan, J Zhou, RK Au-Yeung, KH Sze, D Yang, H Shuai, Y Hou, C Li, X Zhao, VK Poon, SP Leung, ML Yeung, J Yan, G Lu, DY Jin, GF Gao, JF Chan, KY Yuen
    J. Biol. Chem., 2018;0(0):.
    Applications: Western Blot
  7. Experimental infection of dromedaries with Middle East respiratory syndrome-Coronavirus is accompanied by massive ciliary loss and depletion of the cell surface receptor dipeptidyl peptidase 4
    Authors: AK Haverkamp, A Lehmbecker, I Spitzbarth, W Widagdo, BL Haagmans, J Segalés, J Vergara-Al, A Bensaid, JMA van den Br, ADME Osterhaus, W Baumgärtne
    Sci Rep, 2018;8(1):9778.
    Species: Camelid
    Sample Types: Whole Cells
    Applications: ICC
  8. Elevated CD26 Expression by Skin Fibroblasts Distinguishes a Profibrotic Phenotype Involved in Scar Formation Compared to Gingival Fibroblasts
    Authors: W Mah, G Jiang, D Olver, C Gallant-Be, C Wiebe, DA Hart, L Koivisto, H Larjava, L Häkkinen
    Am. J. Pathol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. Identification of sialic acid-binding function for the Middle East respiratory syndrome coronavirus spike glycoprotein
    Authors: W Li, RJG Hulswit, I Widjaja, VS Raj, R McBride, W Peng, W Widagdo, MA Tortorici, B van Dieren, Y Lang, JWM van Lent, JC Paulson, CAM de Haan, RJ de Groot, FJM van Kuppev, BL Haagmans, BJ Bosch
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. First-in-human phase 1 of YS110, a monoclonal antibody directed against CD26 in advanced CD26-expressing cancers
    Authors: E Angevin, N Isambert, V Trillet-Le, B You, J Alexandre, G Zalcman, P Vielh, F Farace, F Valleix, T Podoll, Y Kuramochi, I Miyashita, O Hosono, NH Dang, K Ohnuma, T Yamada, Y Kaneko, C Morimoto
    Br. J. Cancer, 2017;116(9):1126-1134.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  11. Regulation of pulmonary graft-versus-host disease by IL-26+CD26+CD4 T lymphocytes.
    Authors: Ohnuma K, Hatano R, Aune T, Otsuka H, Iwata S, Dang N, Yamada T, Morimoto C
    J Immunol, 2015;194(8):3697-712.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. High secretion of interferons by human plasmacytoid dendritic cells upon recognition of Middle East respiratory syndrome coronavirus.
    Authors: Scheuplein V, Seifried J, Malczyk A, Miller L, Hocker L, Vergara-Alert J, Dolnik O, Zielecki F, Becker B, Spreitzer I, Konig R, Becker S, Waibler Z, Muhlebach M
    J Virol, 2015;89(7):3859-69.
    Species: Human
    Sample Types: Whole Cells
    Applications: Blocking
  13. Generation of a transgenic mouse model of Middle East respiratory syndrome coronavirus infection and disease.
    Authors: Agrawal A, Garron T, Tao X, Peng B, Wakamiya M, Chan T, Couch R, Tseng C
    J Virol, 2015;89(7):3659-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Characterization of dedifferentiating human mature adipocytes from the visceral and subcutaneous fat compartments: fibroblast-activation protein alpha and dipeptidyl peptidase 4 as major components of matrix remodeling.
    Authors: Lessard J, Pelletier M, Biertho L, Biron S, Marceau S, Hould F, Lebel S, Moustarah F, Lescelleur O, Marceau P, Tchernof A
    PLoS ONE, 2015;10(3):e0122065.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Glycosylation of mouse DPP4 plays a role in inhibiting Middle East respiratory syndrome coronavirus infection.
    Authors: Peck K, Cockrell A, Yount B, Scobey T, Baric R, Heise M
    J Virol, 2015;89(8):4696-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  16. Lineage-specific regulation of epigenetic modifier genes in human liver and brain.
    Authors: Weng, Matthias, Natarajan, Karthick, Scholz, Diana, Ivanova, Violeta, Sachinidis, Agapios, Hengstler, Jan G, Waldmann, Tanja, Leist, Marcel
    PLoS ONE, 2014;9(7):e102035.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  17. Adenosine deaminase acts as a natural antagonist for dipeptidyl peptidase 4-mediated entry of the Middle East respiratory syndrome coronavirus.
    Authors: Raj, V Stalin, Smits, Saskia L, Provacia, Lisette, van den Brand, Judith M, Wiersma, Lidewij, Ouwendijk, Werner J, Bestebroer, Theo M, Spronken, Monique, van Amerongen, Geert, Rottier, Peter J, Fouchier, Ron A M, Bosch, Berend J, Osterhaus, Albert D, Haagmans, Bart L
    J Virol, 2014;88(3):1834-8.
    Species: Ferret
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Regulation of somatostatin receptor 4-mediated cytostatic effects by CD26 in malignant pleural mesothelioma.
    Authors: Yamamoto, J, Ohnuma, K, Hatano, R, Okamoto, T, Komiya, E, Yamazaki, H, Iwata, S, Dang, N H, Aoe, K, Kishimoto, T, Yamada, T, Morimoto, C
    Br J Cancer, 2014;110(9):2232-45.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  19. In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection.
    Authors: Eckerle I, Muller M, Kallies S, Gotthardt D, Drosten C
    Virol J, 2013;10(0):359.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  20. CD26 expression on T-anaplastic large cell lymphoma (ALCL) line Karpas 299 is associated with increased expression of versican and MT1-MMP and enhanced adhesion.
    Authors: Havre, Pamela A, Dang, Long H, Ohnuma, Kei, Iwata, Satoshi, Morimoto, Chikao, Dang, Nam H
    BMC Cancer, 2013;13(0):517.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Distinct gene expression-defined classes of gastrointestinal stromal tumor.
    Authors: Yamaguchi U, Nakayama R, Honda K, Ichikawa H, Hasegawa T, Shitashige M, Ono M, Shoji A, Sakuma T, Kuwabara H, Shimada Y, Sasako M, Shimoda T, Kawai A, Hirohashi S, Yamada T
    J. Clin. Oncol., 2008;26(25):4100-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. Lymphatic-specific expression of dipeptidyl peptidase IV and its dual role in lymphatic endothelial function.
    Authors: Shin JW, Jurisic G, Detmar M
    Exp. Cell Res., 2008;314(16):3048-56.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Trypsin Treatment Unlocks Barrier for Zoonotic Bat Coronavirus Infection.
    Authors: Menachery V, Dinnon K, Yount B, McAnarney E, Gralinski L, Hale A, Graham R, Scobey T, Anthony S, Wang L, Graham B, Randell S, Lipkin W, Baric R
    J Virol, 0;94(5):.
    Species: Human
    Sample Types: cell culture

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Human DPPIV/CD26 Antibody
By Anonymous on 01/21/2020
Application: IHC Sample Tested: Small intestine Species: Human

IHC-paraffin
working dilution: 1ug/mL
with Bond Refine polymer Detection
rabbit anti-goat IgG as a Linker antibody
epitope retrieval: basic (BOND ER2)


Human DPPIV/CD26 Antibody
By Anonymous on 08/11/2016
Application: IP Sample Tested: Heparin Plasma Species: Mouse