Human DPPIV/CD26 Antibody

Name: Human DPPIV/CD26 Antibody
Brand: R&D Systems
Rating: 5 (1 reviews)

(14 citations)

(1 Review)

Datasheet / CoA / SDS

Western Blot

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Western Blot

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Immunocytochemistry

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Immunohistochemistry

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Simple Western

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Product Details

Citations (14)

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Species Reactivity

Human
Specificity

Detects human DPPIV/CD26 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse DPPIV is observed.
Source

Polyclonal Goat IgG
Purification

Antigen Affinity-purified
Immunogen

Mouse myeloma cell line NS0-derived recombinant human DPPIV/CD26 (Catalog # 1180-SE)
Asp34-Pro766
Accession # Q53TN1
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

0.2 µg/mL

See below
Simple Western

2 µg/mL

See below
Immunohistochemistry

1-15 µg/mL

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Immunocytochemistry

1-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot click image to view larger	Detection of Human DPPIV/CD26 by Western Blot. Western blot shows lysate of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot click image to view larger	Detection of Human DPPIV/CD26 by Western Blot. Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry click image to view larger	DPPIV/CD26 in Human PBMCs. DPPIV/CD26 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry click image to view larger	DPPIV/CD26 in Human Psoriatic Skin. DPPIV/CD26 was detected in immersion fixed paraffin-embedded sections of human psoriatic skin using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Simple Western click image to view larger	Detection of Human DPPIV/CD26 by Simple Western^TM. Simple Western lane view shows lysates of LoVo human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 168 kDa (as indicated) using 2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DPPIV/CD26

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides. It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain. The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.

DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a cofactor for entry of HIV in CD4⁺ cells. It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans. It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine. It degrades peptide hormones such as glucagon. It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis.

Long Name:

Dipeptidyl-peptidase IV

Entrez Gene IDs:

1803 (Human); 13482 (Mouse); 102133935 (Cynomolgus Monkey)

Alternate Names:

ADABP; ADCP-2; ADCP2DPP IV; Adenosine deaminase complexing protein 2TP103; CD26 antigen; CD26; CD26T-cell activation antigen CD26; dipeptidyl peptidase 4; Dipeptidyl peptidase IV; dipeptidylpeptidase 4; dipeptidyl-peptidase 4; dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2); DPP4; DPPIV; EC 3.4.14.5

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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Species	Applications	Sample Type
First-in-human phase 1 of YS110, a monoclonal antibody directed against CD26 in advanced CD26-expressing cancers Authors: E Angevin, N Isambert, V Trillet-Le, B You, J Alexandre, G Zalcman, P Vielh, F Farace, F Valleix, T Podoll, Y Kuramochi, I Miyashita, O Hosono, NH Dang, K Ohnuma, T Yamada, Y Kaneko, C Morimoto Br. J. Cancer, 2017;116(9):1126-1134. Species: Human Sample Type: Whole Tissue Application: IHC Identification of sialic acid-binding function for the Middle East respiratory syndrome coronavirus spike glycoprotein Authors: W Li, RJG Hulswit, I Widjaja, VS Raj, R McBride, W Peng, W Widagdo, MA Tortorici, B van Dieren, Y Lang, JWM van Lent, JC Paulson, CAM de Haan, RJ de Groot, FJM van Kuppev, BL Haagmans, BJ Bosch Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. Species: Human Sample Type: Whole Cells Application: Flow High secretion of interferons by human plasmacytoid dendritic cells upon recognition of Middle East respiratory syndrome coronavirus. Authors: Scheuplein V, Seifried J, Malczyk A, Miller L, Hocker L, Vergara-Alert J, Dolnik O, Zielecki F, Becker B, Spreitzer I, Konig R, Becker S, Waibler Z, Muhlebach M J Virol, 2015;89(7):3859-69. Species: Human Sample Type: Whole Cells Application: Blocking Characterization of dedifferentiating human mature adipocytes from the visceral and subcutaneous fat compartments: fibroblast-activation protein alpha and dipeptidyl peptidase 4 as major components of matrix remodeling. Authors: Lessard J, Pelletier M, Biertho L, Biron S, Marceau S, Hould F, Lebel S, Moustarah F, Lescelleur O, Marceau P, Tchernof A PLoS ONE, 2015;10(3):e0122065. Species: Human Sample Type: Cell Lysates Application: WB Generation of a transgenic mouse model of Middle East respiratory syndrome coronavirus infection and disease. Authors: Agrawal A, Garron T, Tao X, Peng B, Wakamiya M, Chan T, Couch R, Tseng C J Virol, 2015;89(7):3659-70. Species: Human Sample Type: Cell Lysates Application: WB Regulation of pulmonary graft-versus-host disease by IL-26+CD26+CD4 T lymphocytes. Authors: Ohnuma K, Hatano R, Aune T, Otsuka H, Iwata S, Dang N, Yamada T, Morimoto C J Immunol, 2015;194(8):3697-712. Species: Mouse Sample Type: Whole Tissue Application: IHC - paraffin Glycosylation of mouse DPP4 plays a role in inhibiting Middle East respiratory syndrome coronavirus infection. Authors: Peck K, Cockrell A, Yount B, Scobey T, Baric R, Heise M J Virol, 2015;89(8):4696-9. Species: Human Sample Type: Whole Cells Application: IHC Not Specified Adenosine deaminase acts as a natural antagonist for dipeptidyl peptidase 4-mediated entry of the Middle East respiratory syndrome coronavirus. Authors: Raj, V Stalin, Smits, Saskia L, Provacia, Lisette, van den Brand, Judith M, Wiersma, Lidewij, Ouwendijk, Werner J, Bestebroer, Theo M, Spronken, Monique, van Amerongen, Geert, Rottier, Peter J, Fouchier, Ron A M, Bosch, Berend J, Osterhaus, Albert D, Haagmans, Bart L J Virol, 2014;88(3):1834-8. Species: Ferret Sample Type: Whole Cells Application: Flow Lineage-specific regulation of epigenetic modifier genes in human liver and brain. Authors: Weng, Matthias, Natarajan, Karthick, Scholz, Diana, Ivanova, Violeta, Sachinidis, Agapios, Hengstler, Jan G, Waldmann, Tanja, Leist, Marcel PLoS ONE, 2014;9(7):e102035. Species: Human Sample Type: Whole Cells Application: ICC Regulation of somatostatin receptor 4-mediated cytostatic effects by CD26 in malignant pleural mesothelioma. Authors: Yamamoto, J, Ohnuma, K, Hatano, R, Okamoto, T, Komiya, E, Yamazaki, H, Iwata, S, Dang, N H, Aoe, K, Kishimoto, T, Yamada, T, Morimoto, C Br J Cancer, 2014;110(9):2232-45. Species: Human Sample Type: Cell Lysates Application: Immunprecipitation CD26 expression on T-anaplastic large cell lymphoma (ALCL) line Karpas 299 is associated with increased expression of versican and MT1-MMP and enhanced adhesion. Authors: Havre, Pamela A, Dang, Long H, Ohnuma, Kei, Iwata, Satoshi, Morimoto, Chikao, Dang, Nam H BMC Cancer, 2013;13(0):517. Species: Human Sample Type: Cell Lysates Application: WB In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection. Authors: Eckerle I, Muller M, Kallies S, Gotthardt D, Drosten C Virol J, 2013;10(0):359. Species: Human Sample Type: Whole Tissue Application: IF Lymphatic-specific expression of dipeptidyl peptidase IV and its dual role in lymphatic endothelial function. Authors: Shin JW, Jurisic G, Detmar M Exp. Cell Res., 2008;314(16):3048-56. Species: Human Sample Type: Cell Lysates Application: WB Distinct gene expression-defined classes of gastrointestinal stromal tumor. Authors: Yamaguchi U, Nakayama R, Honda K, Ichikawa H, Hasegawa T, Shitashige M, Ono M, Shoji A, Sakuma T, Kuwabara H, Shimada Y, Sasako M, Shimoda T, Kawai A, Hirohashi S, Yamada T J. Clin. Oncol., 2008;26(25):4100-8. Species: Human Sample Type: Whole Tissue Application: IHC Paraffin-embedded Expand to show all Citations

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CTS008 17

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Human DPPIV/CD26 Antibody

Certificate of Analysis Lookup

Certificate of Analysis Lookup

Certificate of Analysis Request Form

FAQs

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

Antigen Retrieval Reagent-Universal

Antigen Retrieval Reagent-Basic

Mounting Medium

Antigen Retrieval Reagent Sampler (50 mL each)

Antigen Retrieval Reagent-Acidic

NorthernLights Guard Mounting Media

VisUCyte Antigen Retrieval Reagent-Universal

VisUCyte Antigen Retrieval Reagent-Acidic

VisUCyte Antigen Retrieval Reagent-Basic

Normal Goat IgG Control

Reconstitution Buffer 1 (PBS)

Goat IgG HRP-conjugated Antibody

Goat IgG HRP-conjugated Antibody

Goat IgG (H+L) PE-conjugated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Goat IgG Horseradish Peroxidase-conjugated Antibody

Goat IgG (H+L) APC-conjugated Antibody

Donkey Anti-Goat IgG Antibody

Goat IgG VisUCyte HRP Polymer

Donkey Anti-Goat IgG Biotinylated Antibody

Goat IgG (H+L) Fluorescein-conjugated Antibody

Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

Chicken Anti-Goat IgG Biotinylated Antibody

Rabbit Anti-Goat IgG Biotinylated Antibody

Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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