Detection of Human DUX4 by Western Blot.
Western blot shows lysates of C2C12 mouse myoblast cell line transfected with human DUX4 untreated (-) or treated (+) with Doxycycline. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human DUX4 Monoclonal Antibody (Catalog # MAB9535) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for DUX4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
DUX4 in Human Testis.
DUX4 was detected in immersion fixed paraffin-embedded sections of human testis using Rabbit Anti-Human DUX4 Monoclonal Antibody (Catalog # MAB9535) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human DUX4 by Simple WesternTM.
Simple Western lane view shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human DUX4 and untreated (-) or treated (+) with Doxycycline, loaded at 0.2 mg/mL. A specific band was detected for DUX4 at approximately 62 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Human DUX4 Monoclonal Antibody (Catalog # MAB9535). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
homeobox 4 (aka DUX4) is a protein encoded by the DUX4 gene. This gene is
located within a D4Z4 repeat array in chromosome 4q35. Each D4Z4 repeat unit
has an open reading frame, DUX4, that contains two homeoboxes. Unregulated
expression of DUX4 in muscle cells is the cause of facioscapulohumeral muscular
dystrophy (FSHD), a common form of muscular dystrophy in adults that is one of
the most prevalent genetic diseases of muscle.
Double Homeobox 4
Entrez Gene IDs:
double homeobox 4; double homeobox protein 10; double homeobox protein 4; double homeobox protein 4/10; double homeobox protein DUX10; DUX10; DUX4; DUX4L1
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