Human E-Selectin/CD62E Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    This antibody binds to CHO cells transfected with human E-Selectin. It does not bind to COS cells transfected with human PECAM‑1, P‑Selectin, L‑Selectin, VCAM‑1, or ICAM‑1.
  • Source
    Monoclonal Mouse IgG1 Clone # BBIG-E1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Activated HUVEC human umbilical vein endothelial cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)
  • Immunoprecipitation
    Pigott, R. et al. (1991) J. Immunol. 147:130.
  • Adhesion Blockade
    The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human E‑Selectin (Catalog # ADP1, 2 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Immunofluorescence

    Pigott, R. et al. (1991) J. Immunol. 147:130.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
E‑Selectin/CD62E in HUVECs. E‑Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells stimulated with Recombinant Human TNF-alpha (Catalog # 210-TA) using Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA26) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Selectin/CD62E
E-Selectin, also known as ELAM-1, is a transmembrane glycoprotein that is transiently expressed on activated vascular endothelial cells. E-Selectin binds to sialyated, fucosylated molecules on neutrophils, monocytes, and a subset of memory T cells.
  • Entrez Gene IDs:
    6401 (Human); 20339 (Mouse); 25544 (Rat)
  • Alternate Names:
    CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. A Glycovariant of Human CD44 is Characteristically Expressed on Human Mesenchymal Stem Cells
    Stem Cells, 2016;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Annexin A8 controls leukocyte recruitment to activated endothelial cells via cell surface delivery of CD63.
    Authors: Poeter M, Brandherm I, Rossaint J, Rosso G, Shahin V, Skryabin B, Zarbock A, Gerke V, Rescher U
    Nat Commun, 2014;5(0):3738.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Leukemia Mediated Endothelial Cell Activation Modulates Leukemia Cell Susceptibility to Chemotherapy through a Positive Feedback Loop Mechanism.
    Authors: Pezeshkian, Bahareh, Donnelly, Christop, Tamburo, Kelley, Geddes, Timothy, Madlambayan, Gerard J
    PLoS ONE, 2013;8(4):e60823.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  4. The adaptor molecule Lnk negatively regulates tumor necrosis factor-alpha-dependent VCAM-1 expression in endothelial cells through inhibition of the ERK1 and -2 pathways.
    Authors: Fitau J, Boulday G, Coulon F, Quillard T, Charreau B
    J. Biol. Chem., 2006;281(29):20148-59.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Ligation of ICAM-1 on endothelial cells leads to expression of VCAM-1 via a nuclear factor-kappaB-independent mechanism.
    Authors: Lawson C, Ainsworth M, Yacoub M, Rose M
    J. Immunol., 1999;162(5):2990-6.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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