Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Adhesion Blockade, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, CyTOF-ready

Cited:

Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Blocking, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # BBIG-E1
View all E-Selectin/CD62E Primary Antibodies »

Product Specifications

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Specificity

This antibody binds to CHO cells transfected with human E-Selectin. It does not bind to COS cells transfected with human PECAM‑1, P‑Selectin, L‑Selectin, VCAM‑1, or ICAM‑1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human E‑Selectin/CD62E Antibody

Detection of E-Selectin/CD62E in HUVECs by Flow Cytometry.

Human umbilical cord endothelial cells (HUVECs) were cultured for 6 hours in the presence of 25 ng/mL of rhTNF-alpha (210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA26) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.

E-Selectin/CD62E antibody in HUVECs by Immunocytochemistry (ICC).

E‑Selectin/CD62E in HUVECs.

E-Selectin/CD62E was detected in immersion fixed HUVEC human umbilical vein endothelial cells stimulated with Recombinant Human TNF-alpha (Catalog # 210-TA) using Mouse Anti-Human E-Selectin/CD62E Monoclonal Antibody (Catalog # BBA26) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human E‑Selectin/CD62E Antibody

Application
Recommended Usage

Adhesion Blockade

The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human E‑Selectin (Catalog # ADP1, 2 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 25 µg/mL of the antibody.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells stimulated with Recombinant Human TNF-alpha (Catalog # 210-TA)

Immunofluorescence

Pigott, R. et al. (1991) J. Immunol. 147:130.

Immunoprecipitation

Pigott, R. et al. (1991) J. Immunol. 147:130.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: E-Selectin/CD62E

E-Selectin, also known as ELAM-1, is a transmembrane glycoprotein that is transiently expressed on activated vascular endothelial cells. E-Selectin binds to sialyated, fucosylated molecules on neutrophils, monocytes, and a subset of memory T cells.

Alternate Names

CD62E, ELAM1, LECAM2, SELE

Entrez Gene IDs

6401 (Human); 20339 (Mouse); 25544 (Rat); 102143564 (Cynomolgus Monkey)

Gene Symbol

SELE

Additional E-Selectin/CD62E Products

Product Documents for Human E‑Selectin/CD62E Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human E‑Selectin/CD62E Antibody

For research use only

Citations for Human E‑Selectin/CD62E Antibody

Customer Reviews for Human E‑Selectin/CD62E Antibody

There are currently no reviews for this product. Be the first to review Human E‑Selectin/CD62E Antibody and earn rewards!

Have you used Human E‑Selectin/CD62E Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies