Human EGR1 Antibody

Catalog # Availability Size / Price Qty
AF2818
AF2818-SP
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews

Human EGR1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human EGR1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human EGR1
Gln282-Ser433
Accession # P18146
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human EGR1
Immunocytochemistry
5-15 µg/mL
Immersion fixed PC-3 human prostate cancer cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGR1

Early Growth Response 1, (EGR1) belongs to the EGR family of zinc finger transcription factors and regulates the expression of several tumor suppressors. Its expression is induced by diverse signals that initiate growth and differentiation. EGR1 suppresses or activates cell growth depending on the tumor type. Within the region that includes its three zinc finger domains, human EGR1 shares 99% aa sequence identity with mouse and rat EGR1.

Long Name
Early Growth Response 1
Entrez Gene IDs
1958 (Human)
Alternate Names
AT225Transcription factor ETR103; early growth response 1; early growth response protein 1; EGR1; EGR-1; G0S30; KROX24; KROX-24; Nerve growth factor-induced protein A; NGFIA; NGFI-ATranscription factor Zif268; TIS8; ZIF-268; Zinc finger protein Krox-24; ZNF225; ZNF225Zinc finger protein 225

Product Datasheets

Citations for Human EGR1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Transcriptomic analysis reveals cell apoptotic signature modified by heparanase in melanoma cells
    Authors: T Song, D Spillmann
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Expression of early growth response-1 in colorectal cancer and its relation to tumor cell proliferation and apoptosis.
    Authors: Myung D, Park Y, Kim N, Chung C, Park H, Kim J, Cho S, Lee W, Lee J, Joo Y
    Oncol Rep, 2014;31(2):788-94.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Notch signaling influences neuroprotective and proliferative properties of mature Muller glia.
    Authors: Ghai K, Zelinka C, Fischer AJ
    J. Neurosci., 2010;30(8):3101-12.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC
  4. Mitogen-activated protein kinase-signaling regulates the ability of Muller glia to proliferate and protect retinal neurons against excitotoxicity.
    Authors: Fischer AJ, Scott MA, Ritchey ER, Sherwood P
    Glia, 2009;57(14):1538-52.
    Species: Chicken
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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