Human Endorepellin/Perlecan Antibody

Catalog # Availability Size / Price Qty
AF2364
AF2364-SP
Endorepellin/Perlecan in Human Skin.
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Product Details
Citations (3)
FAQs
Supplemental Products
Reviews (3)

Human Endorepellin/Perlecan Antibody Summary

Species Reactivity
Human
Specificity
Detects human Endorepellin/Perlecan in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Endorepellin/Perlecan
Arg3684-Ser4391 (Ser4003Thr)
Accession # P98160
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Endorepellin/Perlecan
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry Endorepellin/Perlecan in Human Skin. View Larger

Endorepellin/Perlecan in Human Skin. Endorepellin/Perlecan was detected in immersion fixed paraffin-embedded sections of human skin using 5 µg/mL Goat Anti-Human Endorepellin/
Perlecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2364) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endorepellin/Perlecan

Endorepellin is the C-terminal domain of the heparan sulfate proteoglycan perlecan, which is a modular protein found in basement membranes. Endorepellin has been found to be an inhibitor of angiogenesis that blocks endothelial cell adhesion to fibronectin and type I collagen.

Entrez Gene IDs
3339 (Human); 15530 (Mouse)
Alternate Names
basement membrane-specific heparan sulfate proteoglycan core protein; endorepellin (domain V region); Endorepellin; heparan sulfate proteoglycan 2; HSPG; HSPG2; perlecan proteoglycan; Perlecan; PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia); PRCAN; SJA; SJS; SJS1

Product Datasheets

Citations for Human Endorepellin/Perlecan Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop.
    Authors: Ohlund D, Franklin O, Lundberg E, Lundin C, Sund M
    BMC Cancer, 2013;13(0):154.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. A fragment of the LG3 peptide of endorepellin is present in the urine of physically active mining workers: a potential marker of physical activity.
    Authors: Parker TJ, Sampson DL, Broszczak D
    PLoS ONE, 2012;7(3):e33714.
    Species: Human
    Sample Types: Urine
    Applications: Western Blot
  3. Endorepellin, the angiostatic module of perlecan, interacts with both the alpha2beta1 integrin and vascular endothelial growth factor receptor 2 (VEGFR2): a dual receptor antagonism.
    Authors: Goyal A, Pal N, Concannon M, Paul M, Doran M, Poluzzi C, Sekiguchi K, Whitelock JM, Neill T, Iozzo RV
    J. Biol. Chem., 2011;286(29):25947-62.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Endorepellin/Perlecan Antibody

Average Rating: 3.7 (Based on 3 Reviews)

5 Star
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4 Star
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3 Star
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Human Endorepellin/Perlecan Antibody
By Anonymous on 03/11/2019
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human Endorepellin/Perlecan Antibody
By Anonymous on 11/08/2018
Application: ELISA Sample Tested: Serum and Plasma Species: Human,Mouse

Human Endorepellin/Perlecan Antibody
By Anonymous on 10/31/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human