Human Endorepellin/Perlecan Antibody Summary
Accession # P98160
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Endorepellin/Perlecan in Human Skin.
Endorepellin/Perlecan was detected in immersion fixed paraffin-embedded sections of human skin using 5 µg/mL Goat Anti-Human Endorepellin/
Perlecan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2364) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Endorepellin is the C-terminal domain of the heparan sulfate proteoglycan perlecan, which is a modular protein found in basement membranes. Endorepellin has been found to be an inhibitor of angiogenesis that blocks endothelial cell adhesion to fibronectin and type I collagen.
Citations for Human Endorepellin/Perlecan Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop.
Authors: Ohlund D, Franklin O, Lundberg E, Lundin C, Sund M
BMC Cancer, 2013;13(0):154.
Sample Types: Whole Cells
A fragment of the LG3 peptide of endorepellin is present in the urine of physically active mining workers: a potential marker of physical activity.
Authors: Parker TJ, Sampson DL, Broszczak D
PLoS ONE, 2012;7(3):e33714.
Sample Types: Urine
Applications: Western Blot
Endorepellin, the angiostatic module of perlecan, interacts with both the alpha2beta1 integrin and vascular endothelial growth factor receptor 2 (VEGFR2): a dual receptor antagonism.
Authors: Goyal A, Pal N, Concannon M, Paul M, Doran M, Poluzzi C, Sekiguchi K, Whitelock JM, Neill T, Iozzo RV
J. Biol. Chem., 2011;286(29):25947-62.
Sample Types: Cell Lysates
Applications: Western Blot
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