Human Endothelin-1 C-Terminus Antibody

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Endothelin‑1 in HUVEC Human Cells.
2 Images
Product Details
Citations (2)
Supplemental Products

Human Endothelin-1 C-Terminus Antibody Summary

Species Reactivity
Detects human Endothelin‑1 C-Terminus. Recognizes the C-terminal region of mature Endothelin-1, -2, and -3 (amino acid residues 15‑21).
Monoclonal Rat IgG2B Clone # 3G10
Protein A or G purified from hybridoma culture supernatant
Human Endothelin-1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Endothelin‑1 C-Terminus Monoclonal Antibody (Catalog # MAB34401).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Endothelin Pan Specific DuoSet ELISA Kit (Catalog # DY1160) for convenient development of a sandwich ELISA or the Endothelin-1 Quantikine ELISA Kit (Catalog # DET100) for a complete optimized ELISA.

8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Endothelin-1 antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

Endothelin‑1 in HUVEC Human Cells. Endothelin-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB3440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Human Endothelin‑1 ELISA Standard Curve. Recombinant Human Endothelin-1 protein was serially diluted 2-fold and captured by Rat Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB3440) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB34401) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endothelin-1

Endothelin-1, -2, and -3 are encoded by three separate genes as prepropolypeptides that are cleaved to yield inactive big endothelins. Big Endothelins are further cleaved to generate the 21 amino acid bioactive mature peptides. Endothelin-1 has potent vasoconstricting and angiogenic activity that is mediated through the 7TM receptors, ET-A and ET-B. Endothelin-1, -2, and -3 share identical amino acid sequences at their C-termini (residues 15‑21).

Entrez Gene IDs
1906 (Human); 13614 (Mouse); 24323 (Rat)
Alternate Names
ARCND3; EDN1; endothelin 1; Endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1; QME

Product Datasheets

You must select a language.


Citations for Human Endothelin-1 C-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Increased mortality with elevated plasma endothelin-1 in acute heart failure: an ASCEND-HF biomarker substudy.
    Authors: Perez A, Grodin J, Wu Y, Hernandez A, Butler J, Metra M, Felker G, Voors A, McMurray J, Armstrong P, Starling R, O'Connor C, Tang W
    Eur J Heart Fail, 2015-12-14;18(3):290-7.
    Species: Human
    Sample Types: Plasma
  2. Expression of endothelial nitric oxide synthase and endothelin-1 in skin tissue from amputated limbs of patients with complex regional pain syndrome.
    Authors: Groeneweg JG, Antonissen CH, Huygen FJ
    Mediators Inflamm., 2008-01-01;2008(0):680981.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Endothelin-1 C-Terminus Antibody

There are currently no reviews for this product. Be the first to review Human Endothelin-1 C-Terminus Antibody and earn rewards!

Have you used Human Endothelin-1 C-Terminus Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review