Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunocytochemistry

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 3G10
View all Endothelin-1 Primary Antibodies »

Product Specifications

Immunogen

Human Endothelin-1

Specificity

Detects human Endothelin‑1 C-Terminus. Recognizes the C-terminal region of mature Endothelin-1, -2, and -3 (amino acid residues 15‑21).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human Endothelin‑1 C-Terminus Antibody

Endothelin-1 antibody in HUVEC Human Cells by Immunocytochemistry (ICC).

Endothelin‑1 in HUVEC Human Cells.

Endothelin-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB3440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Human Endothelin-1 Antibody in ELISA Standard Curve.

Human Endothelin‑1 ELISA Standard Curve.

Recombinant Human Endothelin-1 protein was serially diluted 2-fold and captured by Rat Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB3440) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Endothelin-1 C-Terminus Monoclonal Antibody (Catalog # MAB34401) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Detection of Human Endothelin-1 by Immunohistochemistry

Detection of Human Endothelin-1 by Immunohistochemistry

Double staining for CD31 and ET-1 in skin tissue fromthe amputated leg of CRPS patient B. CD31-positive blood vessels are stainedred and ET-1-positive cells are stained blue. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/18663383), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Endothelin‑1 C-Terminus Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Endothelin‑1 C-Terminus Monoclonal Antibody (Catalog # MAB34401).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Endothelin Pan Specific DuoSet ELISA Kit (Catalog # DY1160) for convenient development of a sandwich ELISA or the Endothelin-1 Quantikine ELISA Kit (Catalog # DET100) for a complete optimized ELISA.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Endothelin-1

Endothelin-1, -2, and -3 are encoded by three separate genes as prepropolypeptides that are cleaved to yield inactive big endothelins. Big Endothelins are further cleaved to generate the 21 amino acid bioactive mature peptides. Endothelin-1 has potent vasoconstricting and angiogenic activity that is mediated through the 7TM receptors, ET-A and ET-B. Endothelin-1, -2, and -3 share identical amino acid sequences at their C-termini (residues 15‑21).

Alternate Names

ARCND3, EDN1, ET1, HDLCQ7, PPET1, QME

Entrez Gene IDs

1906 (Human); 13614 (Mouse); 24323 (Rat)

Gene Symbol

EDN1

Additional Endothelin-1 Products

Product Documents for Human Endothelin‑1 C-Terminus Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Endothelin‑1 C-Terminus Antibody

For research use only

Citations for Human Endothelin‑1 C-Terminus Antibody

Customer Reviews for Human Endothelin‑1 C-Terminus Antibody

There are currently no reviews for this product. Be the first to review Human Endothelin‑1 C-Terminus Antibody and earn rewards!

Have you used Human Endothelin‑1 C-Terminus Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies