Detects human Ephrin-A5 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Ephrin-A1, rmEphrin-A2, recombinant human (rh) Ephrin-A3, rhEphrin-A4, rhEphrin-B3, rmEphrin-A4, rmEphrin-B1 and rmEphrin-B2 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human Ephrin-A5 Gln21-Asn203 Accession # P52803
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Recombinant Human Ephrin-A5 Fc Chimera (Catalog # 374-EA)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Ephrin‑A5 in Human Melanoma. Ephrin‑A5 was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human Ephrin‑A5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3743) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Ephrin-A5, also known as AL-1, RAGS, LERK-7, and EFL-5 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A5 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphA8 (2, 3). The extracellular domains of murine and human Ephrin-A5 share 99% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).
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