Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1090209
View all Exosome Component 9 Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide
Accession # Q06265

Specificity

Detects a synthetic peptide specific for hEXOSC9 around amino acid 300 in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Exosome Component 9 Antibody

Detection of Exosome Component 9 in Human Colon Cancer.

Exosome Component 9 was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human Exosome Component 9 Monoclonal Antibody (Catalog # MAB11616) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Exosome Component 9 in U251‑MG Human Cell Line.

Exosome Component 9 was detected in immersion fixed U251‑MG human malignant glioblastoma cell line using Mouse Anti-Human Exosome Component 9 Monoclonal Antibody (Catalog # MAB11616) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Exosome Component 9 Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed U251-MG human malignant glioblastoma cell line

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Exosome Component 9

Exosome Component 9 (EXOSC9) is a 49 kDa protein and one of the six core proteins of the RNA exosome complex. It is known that EXOSC9 is necessary for the normal differentiation of epidermal tissues. EXOSC9 is also necessary for P-body formation, stress resistance and tumorigenicity in cancers. Depletion of EXOSC9 attenuates stress resistance and P-body formation in cancer cells and higher EXOSC9 activity correlates with poor prognosis in some types of cancers.

References

  1. Yoshino S, Matsui Y, Fukui Y, Seki M, Yamaguchi K, Kanamori A, Saitoh Y, Shimamura T, Suzuki Y, Furukawa Y, Kaneko S, Seiki M, Murakami Y, Inoue JI, Sakamoto T. EXOSC9 depletion attenuates P-body formation, stress resistance, and tumorigenicity of cancer cells. Sci Rep. 2020 Jun 9;10(1):9275. doi: 10.1038/s41598-020-66455-2. PMID: 32518284; PMCID: PMC7283315. 
  2. Quttina M, Waiters KD, Khan AF, Karami S, Peidl AS, Babajide MF, Pennington J, Merchant FA, Bawa-Khalfe T. Exosc9 Initiates SUMO-Dependent lncRNA TERRA Degradation to Impact Telomeric Integrity in Endocrine Therapy Insensitive Hormone Receptor-Positive Breast Cancer. Cells. 2023 Oct 20;12(20):2495. doi: 10.3390/cells12202495. PMID: 37887339; PMCID: PMC10605189.

Alternate Names

p5, p6, PCH1D, PM/Scl-75, PMSCL1, RRP45, Rrp45p

Entrez Gene IDs

5393 (Human); 50911 (Mouse); 294975 (Rat)

Gene Symbol

EXOSC9

UniProt

Q06265 (Human)

Additional Exosome Component 9 Products

Product Documents for Human Exosome Component 9 Antibody

Certificate of Analysis

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Product Specific Notices for Human Exosome Component 9 Antibody

For research use only

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Protocols

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