Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 965616
View all FABP1/L-FABP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FABP1/L-FABP
Met1-Ile127
Accession # P07148

Specificity

Detects human FABP-1/L-FABP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human FABP1/L‑FABP Antibody

Detection of Human FABP1/L-FABP antibody by Western Blot.

Detection of Human FABP1/L‑FABP by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human colon tissue, and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FABP1/L-FABP Monoclonal Antibody (Catalog # MAB29644) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP1/L-FABP antibody in Human iPS Cells by Immunocytochemistry (ICC).

FABP1/L‑FABP in Human iPS Cells.

FABP1/L-FABP was detected in immersion fixed human induced pluripotent stem (iPS) cells differentiated to hepatocytes using Mouse Anti-Human FABP1/L-FABP Monoclonal Antibody (Catalog # MAB29644) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human FABP1/L‑FABP Antibody

Application
Recommended Usage

Immunocytochemistry

2-25 µg/mL
Sample: Immersion fixed human induced pluripotent stem cells differentiated to hepatocytes

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Human colon tissue, and Human kidney tissue

Reviewed Applications

Read 1 review rated 5 using MAB29644 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FABP1/L-FABP

FABP1, also known as liver FABP (L-FABP, Z-protein and squalene-and sterol-carrier protein [SCP]) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney and lung. FABP1 binds free fatty acids and their co-enzyme A derivatives and may be involved in intracellular lipid transport.

Long Name

Fatty Acid-Binding Protein 1

Alternate Names

L-FABP, LFABP

Entrez Gene IDs

2168 (Human); 14080 (Mouse); 24360 (Rat)

Gene Symbol

FABP1

UniProt

P07148

Additional FABP1/L-FABP Products

Product Documents for Human FABP1/L‑FABP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FABP1/L‑FABP Antibody

For research use only

Related Research Areas

Early Endodermal Lineage Markers
Obesity

Customer Reviews for Human FABP1/L‑FABP Antibody (1)

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  • Human FABP1/L-FABP Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: iPS Cells and Induced pluripotent stem cells
    Species: Human
    Verified Customer | Posted 03/29/2022
    Human FABP1/L‑FABP Antibody MAB29644

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Protocols

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