Human FABP1/L-FABP Antibody

Name: Human FABP1/L-FABP Antibody
Brand: R&D Systems
SKU: MAB29644-SP
Rating: 5 (1 reviews)

Catalog #: MAB29644 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB29644-100
MAB29644-SP

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Detection of Human FABP1/L‑FABP by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FABP1/L-FABP Antibody Summary

Species Reactivity

Human

Specificity

Detects human FABP-1/L-FABP in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 965616

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FABP1/L-FABP
Met1-Ile127
Accession # P07148

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunocytochemistry

2-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human FABP1/L‑FABP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human colon tissue, and human kidney tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FABP1/L-FABP Monoclonal Antibody (Catalog # MAB29644) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FABP1/L-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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FABP1/L‑FABP in Human iPS Cells. FABP1/L-FABP was detected in immersion fixed human induced pluripotent stem (iPS) cells differentiated to hepatocytes using Mouse Anti-Human FABP1/L-FABP Monoclonal Antibody (Catalog # MAB29644) at 2 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP1/L-FABP

FABP1, also known as liver FABP (L-FABP, Z-protein and squalene-and sterol-carrier protein [SCP]) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney and lung. FABP1 binds free fatty acids and their co-enzyme A derivatives and may be involved in intracellular lipid transport.

Long Name

Fatty Acid-Binding Protein 1

Entrez Gene IDs

2168 (Human); 14080 (Mouse); 24360 (Rat)

Alternate Names

FABP1; FABPL; fatty acid binding protein 1, liver; fatty acid-binding protein, liver; LFABP; L-FABP; L-FABPFatty acid-binding protein 1; Liver-type fatty acid-binding protein