Key Product Details

Species Reactivity

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FABP6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FABP6
Ala2-Ala128
Accession # P51161

Specificity

Detects human FABP6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) FABP1, rhFABP2, rhFABP3, rhFABP4, rhFABP5, rhFABP7, and recombinant mouse FABP9 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human FABP6 Antibody

FABP6 antibody in T84 Human Cell Line by Immunocytochemistry (ICC).

FABP6 in T84 Human Cell Line.

FABP6 was detected in immersion fixed T84 human colon carcinoma cell line using Goat Anti-Human FABP6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3880) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human FABP6 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed T84 human colon carcinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human FABP6

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FABP6

Fatty acid binding protein-6 (FABP6), also known as ileal bile acid binding protein (I-BABP) and gastrotropin, is a 15 kDa cytoplasmic protein that belongs to the FABP family. It is expressed in ileal epithelium and multiple other tissues. Alternate transcription promoters generate two transcript variants, encoding a 128 aa and a 177 aa residue protein. Human FABP6 isoform 2 contains 128 amino acid residues and is believed to be acetylated on Ala2. It binds both fatty acids and bile acids and has roles in fatty acid transport and metabolism. The amino acid sequence of human FABP6 is 80%, 78%, and 75% aa identical to that of mouse, canine, and porcine FABP6, respectively.

Long Name

Fatty Acid-Binding Protein 6

Alternate Names

Gastrotropin, I-15P, I-BAP, IBABP, ILBP, ILBP3, ILLBP

Entrez Gene IDs

2172 (Human); 16204 (Mouse); 25440 (Rat)

Gene Symbol

FABP6

UniProt

P51161

Additional FABP6 Products

Product Documents for Human FABP6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FABP6 Antibody

For research use only

Citations for Human FABP6 Antibody

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Protocols

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