Human Fas/TNFRSF6/CD95 Antibody

Clone DX2 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB142
MAB142-SP
Product Details
Citations (4)
FAQs
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Human Fas/TNFRSF6/CD95 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fas in direct ELISAs and Western blots. Does not cross-react with rhCD27, rmCD27, rhCD30, rmCD30, rhCD40, rmCD40, rhDR3, rmDR3, rhHVEM, rmHVEM, rhLymphotoxin beta R, rmLymphotoxin beta R, rhTRAIL R1, rmTRAIL R1, rhTRAIL R2, rmTRAIL R2, rhTRAIL R3, or rhTRAIL R4,
Source
Monoclonal Mouse IgG1 Clone # DX2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
L cells transfected with human Fas
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera (Catalog # 326-FS)
under non-reducing conditions only
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human Alzheimer's disease brain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fas/TNFRSF6/CD95

Fas, also known as APO-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein which binds to monoclonal antibodies that were cytolytic for various human cell lines. In the TNF Receptor superfamily nomenclature, Fas is referred to as TNFRSF6. Human Fas cDNA encodes a 325 amino acid (aa) residue type 1 membrane protein that belongs to the TNF and NGF receptor family. Alternatively spliced cDNAs encoding multiple Fas isoforms, including a soluble form of Fas lacking the transmembrane domain, have also been identified. Fas is highly expressed in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes, and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney, and ovary. The ligand for Fas (FasL) has been identified and shown to be a member of the TNF family of type 2 membrane proteins. FasL is predominantly expressed by activated T‑lymphocytes, NK cells, and in tissues with immune-privileged sites. Soluble FasL can be produced by proteolysis of membrane-associated Fas.

Ligation of Fas by FasL or anti-Fas antibody has been shown to induce apoptotic cell death in Fas-bearing cells. Fas plays a role in the down-regulation of the immune reaction and has been shown to be a key mediator of activation-induced death of activated T lymphocytes. Fas-mediated cell death has also been shown to be important for the deletion of activated or autoreactive B lymphocytes. Besides the perforin/granzyme-based mechanism, the Fas system has been identified as the alternate pathway for CTL-mediated cytotoxicity. FasL has also been shown to function in immunological privileged sites by killing infiltrating Fas-bearing lymphocytes and inflammatory cells.

References
  1. Nagata, S. and P. Golstein (1995) Science 267:1449.
  2. Nagata, S. (1997) Cell 88:355.
  3. Parijs, L. and A.K. Abbas (1996) Current Opinion in Immunol. 8:355.
  4. Green, D.R. and C.F. Ware (1997) Proc. Natl. Acad. Sci. USA 94:5986.
Long Name
Fibroblast-associated
Entrez Gene IDs
355 (Human); 14102 (Mouse); 246097 (Rat); 493881 (Feline)
Alternate Names
Apo-1 antigen; Apo-1; apoptosis antigen 1; Apoptosis-mediating surface antigen FAS; APT1; APT1FASTM; CD95 antigen; CD95; CD95ALPS1A; Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas; FAS1; FASLG receptor; TNFRSF6; TNFRSF6member 6; tumor necrosis factor receptor superfamily member 6

Product Datasheets

Citations for Human Fas/TNFRSF6/CD95 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Invasive trophoblasts stimulate vascular smooth muscle cell apoptosis by a fas ligand-dependent mechanism.
    Authors: Harris LK, Keogh RJ, Wareing M, Baker PN, Cartwright JE, Aplin JD, Whitley GS
    Am. J. Pathol., 2006;169(5):1863-74.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  2. Synergistic induction of apoptosis in breast cancer cells by cotreatment with butyrate and TNF-alpha, TRAIL, or anti-Fas agonist antibody involves enhancement of death receptors' signaling and requires P21(waf1).
    Authors: Chopin V, Slomianny C, Hondermarck H, Le Bourhis X
    Exp. Cell Res., 2004;298(2):560-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  3. Ceramide induces a loss in cytosolic peroxide levels in mononuclear cells.
    Authors: Phillips DC, Griffiths HR
    Biochem. J., 2003;375(0):567-79.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Coexpression of oncostatin M and its receptors and evidence for STAT3 activation in human ovarian carcinomas.
    Authors: Savarese TM, Campbell CL, McQuain C, Mitchell K, Guardiani R, Quesenberry PJ, Nelson BE
    Cytokine, 2002;17(6):324-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay

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