Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all FAT10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FAT10
Met1-Gln165 (Cys160Ser)
Accession # O15205

Specificity

Detects human FAT10 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human Ubiquitin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human FAT10 Antibody

Detection of Human FAT10 antibody by Western Blot.

Detection of Human FAT10 by Western Blot.

Western blot shows lysates of JY human B-lymphoblastoid cell line and COLO 205 human colorectal adenocarcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 18 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human FAT10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8408) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FAT10 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human FAT10 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: JY human B-lymphoblastoid cell line treated Recombinant Human IFN‑ gamma (Catalog # 285-IF)and COLO 205 human colorectal adenocarcinoma cell line (untreated)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FAT10

Human Leukocyte Antigen-F Associated Transcript 10 (FAT10), also known as Ubiquitin D (UBD), is a 165 amino acid (aa) member of the Ubiquitin-like family of proteins. Human FAT10 has a predicted molecular weight of 18.5 kDa and shares 69% aa sequence identity with mouse FAT10. Human FAT10 mRNA is expressed as a single transcript in lymphoblastoid lines and dendritic cells, but more than one mRNA transcript has been identified for murine FAT10. FAT10 can also be induced by IFN-gamma and TNF-alpha in some cell lines. Structurally, FAT10 consists of two Ubiquitin-like domains that are connected by a short linker. Like Ubiquitin, FAT10 has a C-terminal glycine residue that can be used to form isopeptide bonds with target proteins. FAT10 conjugated proteins are targeted to the proteasome where the 26S Proteasome subunit S5a/Angiocidin binds to FAT10 and enables subsequent degradation of the conjugated protein. In addition to S5a/Angiocidin, FAT10 has been shown to interact with Huntingtin, Ataxin-1, MAD2, and NUB1L. FAT10 has been implicated in a number of biological processes such as cell cycle control, antigen presentation, and cytokine response.

Alternate Names

UBD

Entrez Gene IDs

10537 (Human); 24108 (Mouse); 29168 (Rat)

Gene Symbol

UBD

UniProt

O15205

Additional FAT10 Products

Product Documents for Human FAT10 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FAT10 Antibody

For research use only

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