Human Fatty Acid Synthase/FASN Antibody

Catalog # Availability Size / Price Qty
MAB59271
MAB59271-SP

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Detection of Human Fatty Acid Synthase/FASN by Western Blot.
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Human Fatty Acid Synthase/FASN Antibody Summary

Species Reactivity
Human
Specificity
Detects Fatty Acid Synthase/FASN in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) Ephrin-A2 is observed and no cross-reactivity with recombinant human (rh) Ephrin-A3, -B3, rmEphrin-A4, -A5, -B1 or
-B2 is observed.
Source
Monoclonal Mouse IgG2B Clone # 647114
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Fatty Acid Synthase/FASN
Ser10-Cys212
Accession # P49327
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Fatty Acid Synthase/FASN antibody by Western Blot. View Larger

Detection of Human Fatty Acid Synthase/FASN by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, HeLa human cervical epithelial carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 0.25 µg/mL of Mouse Anti-Human Fatty Acid Synthase/FASN Monoclonal Antibody (Catalog # MAB59271) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Fatty Acid Synthase/FASN at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fatty Acid Synthase/FASN

FASN (Fatty Acid Synthase; also FAS) is a cytosolic 270-280 kDa member of the short chain dehydrogenase/reductase family 27X. It is expressed in neurons, skeletal muscle, adipocytes and hepatocytes, and both catalyzes the formation of palmitic acid from acetylCoA and malonylCoA, and likely mediates the transfer of fatty acids to peptides. Human FASN is 2511 amino acids (aa) in length ands contains multiple domains, including a  beta -ketoacyl synthase domain (aa 1-414), an acyl and malonyl transferase domain (aa 429-817), an enoyl reductase domain (aa 1563-1863), a beta -ketoacyl reductase domain (aa 1864-2118) and a thioesterase domain (aa 2207‑2511). FASN exists as an antiparallel homodimer. There is one theoretical alternative start site at Met2073. Over aa 9-212, human FASN shares 92% aa identity with mouse FASN.

Entrez Gene IDs
2194 (Human); 14104 (Mouse)
Alternate Names
EC 2.3.1; EC 2.3.1.85; EC 6.3.3.1; FASN; FASshort chain dehydrogenase/reductase family 27X, member 1; Fatty Acid Synthase; MGC14367; MGC15706; OA-519; SDR27X1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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