Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 800201
View all FBXW7/Cdc4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FBXW7/Cdc4
Lys377-Lys707
Accession # Q969H0

Specificity

Detects human FBXW7/Cdc4 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human FBXW7/Cdc4 Antibody

Detection of Human FBXW7/Cdc4 antibody by Western Blot.

Detection of Human FBXW7/Cdc4 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FBXW7/Cdc4 Monoclonal Antibody (Catalog # MAB7776) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FBXW7/Cdc4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human FBXW7/Cdc4 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Reviewed Applications

Read 3 reviews rated 4 using MAB7776 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FBXW7/Cdc4

F‑box/WD repeat-containing protein 7 (FBXW7), also known as SEL10, CDC4, and AGO, is an approxiimately 110 kDa protein that functions as a substrate recognition component in SCF E3 ubiquitin-protein ligase complexes. FBXW7-containing complexes also contain CUL1, RBX1, and SKP1 and are responsible for the targeting of multiple proteins involved in cell cycle activation. It plays an important role during embryonic development, in the maintenance of neural, oligodendrocyte, and hematopoietic progenitor cells, and in hepatocyte and adipocyte differentiation. FBXW7 is downregulated or mutated in a variety of cancers.  FBXW7 contains one F‑box domain (aa 278-324) and seven tandem WD repeats (aa 378-659). Alternative splicing generates additional isoforms of human FBXW7 that have deletions and/or substitutions of the N-terminal 167 amino acids. Within aa 377-707, human FBXW7 shares 99.7% and 88% aa sequence identity with mouse and rat FBXW7, respectively.

Long Name

F-box and WD Repeat Domain Containing 7

Alternate Names

AGO, Archipelago Homolog, Cdc4, FBW6, Fbw7, FBX30, FBXO30, FBXW6, SCF(Fbw7), SEL10

Entrez Gene IDs

55294 (Human); 50754 (Mouse)

Gene Symbol

FBXW7

UniProt

Q969H0 (Human)

Additional FBXW7/Cdc4 Products

Product Documents for Human FBXW7/Cdc4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FBXW7/Cdc4 Antibody

For research use only

Citations for Human FBXW7/Cdc4 Antibody

Customer Reviews for Human FBXW7/Cdc4 Antibody (3)

4 out of 5
3 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
0%
2 Stars
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1 Stars
0%

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  • Human FBXW7/Cdc4 Antibody
    Name: Jignesh Ganeshbhai Patel
    Application: Western Blot
    Sample Tested: HeLa cells and Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 09/10/2018
  • Human FBXW7/Cdc4 Antibody
    Name: Jignesh Patel
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: THP-1 human acute monocytic leukemia cell line and HeLa cells
    Species: Human
    Verified Customer | Posted 09/10/2018
  • Human FBXW7/Cdc4 Antibody
    Name: Jignesh Patel
    Application: Western Blot
    Sample Tested: THP-1 human acute monocytic leukemia cell line and HeLa cells
    Species: Human
    Verified Customer | Posted 08/21/2018

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FAQs

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Associated Pathways

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