Human FBXW7/Cdc4 Antibody

Catalog # Availability Size / Price Qty
MAB7776
MAB7776-SP
Detection of Human FBXW7/Cdc4 by Western Blot.
1 Image
Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (3)

Human FBXW7/Cdc4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FBXW7/Cdc4 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 800201
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FBXW7/Cdc4
Lys377-Lys707
Accession # Q969H0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human FBXW7/Cdc4 antibody by Western Blot. View Larger

Detection of Human FBXW7/Cdc4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FBXW7/Cdc4 Monoclonal Antibody (Catalog # MAB7776) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FBXW7/Cdc4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FBXW7/Cdc4

F‑box/WD repeat-containing protein 7 (FBXW7), also known as SEL10, CDC4, and AGO, is an approxiimately 110 kDa protein that functions as a substrate recognition component in SCF E3 ubiquitin-protein ligase complexes. FBXW7-containing complexes also contain CUL1, RBX1, and SKP1 and are responsible for the targeting of multiple proteins involved in cell cycle activation. It plays an important role during embryonic development, in the maintenance of neural, oligodendrocyte, and hematopoietic progenitor cells, and in hepatocyte and adipocyte differentiation. FBXW7 is downregulated or mutated in a variety of cancers.  FBXW7 contains one F‑box domain (aa 278-324) and seven tandem WD repeats (aa 378-659). Alternative splicing generates additional isoforms of human FBXW7 that have deletions and/or substitutions of the N-terminal 167 amino acids. Within aa 377-707, human FBXW7 shares 99.7% and 88% aa sequence identity with mouse and rat FBXW7, respectively.

Long Name
F-box and WD Repeat Domain Containing 7
Entrez Gene IDs
55294 (Human); 50754 (Mouse)
Alternate Names
AGO; AGOFBXO30; Archipelago Homolog; archipelago, Drosophila, homolog of; Cdc4; CDC4FLJ16457; F-box and WD repeat domain containing 7; F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila); F-box and WD-40 domain-containing protein 7; F-box protein FBW7; F-box protein FBX30; F-box protein SEL-10; F-box/WD repeat-containing protein 7; FBW6; Fbw7; FBW7hCdc4; FBX30; FBX30FLJ11071; FBXO30; FBXW6; FBXW7; hAgo; homolog of C elegans sel-10; SCF(Fbw7); SEL10; SEL-10DKFZp686F23254; SEL10FBW6

Product Datasheets

Citations for Human FBXW7/Cdc4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Ehrlichia chaffeensis�TRP120-mediated ubiquitination and proteasomal degradation of tumor suppressor FBW7 increases oncoprotein stability and promotes infection
    Authors: JY Wang, B Zhu, LL Patterson, MR Rogan, CE Kibler, JW McBride
    PLoS Pathog., 2020;16(4):e1008541.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Inactivation of EWS reduces PGC-1alpha protein stability and mitochondrial homeostasis.
    Authors: Park J, Kang H, Lee Y, Kang H, Kim J, Chung J, Chang J, McPherron A, Lee S
    Proc Natl Acad Sci U S A, 2015;112(19):6074-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human FBXW7/Cdc4 Antibody

Average Rating: 4 (Based on 3 Reviews)

5 Star
0%
4 Star
100%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human FBXW7/Cdc4 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human FBXW7/Cdc4 Antibody
By Anonymous on 09/10/2018
Application: WB Sample Tested: Blood mononuclear cells (PBMCs),HeLa cells Species: Human

Human FBXW7/Cdc4 Antibody
By Anonymous on 09/10/2018
Application: ICC/IF Sample Tested: HeLa cells,THP-1 human acute monocytic leukemia cell line Species: Human

Human FBXW7/Cdc4 Antibody
By Anonymous on 08/21/2018
Application: WB Sample Tested: HeLa cells,THP-1 human acute monocytic leukemia cell line Species: Human