Human FBXW7/Cdc4 Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human FBXW7/Cdc4 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 800201
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human FBXW7/Cdc4
    Lys377-Lys707
    Accession # Q969H0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human FBXW7/Cdc4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human FBXW7/Cdc4 Monoclonal Antibody (Catalog # MAB7776) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FBXW7/Cdc4 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FBXW7/Cdc4
F‑box/WD repeat-containing protein 7 (FBXW7), also known as SEL10, CDC4, and AGO, is an approxiimately 110 kDa protein that functions as a substrate recognition component in SCF E3 ubiquitin-protein ligase complexes. FBXW7-containing complexes also contain CUL1, RBX1, and SKP1 and are responsible for the targeting of multiple proteins involved in cell cycle activation. It plays an important role during embryonic development, in the maintenance of neural, oligodendrocyte, and hematopoietic progenitor cells, and in hepatocyte and adipocyte differentiation. FBXW7 is downregulated or mutated in a variety of cancers.  FBXW7 contains one F‑box domain (aa 278-324) and seven tandem WD repeats (aa 378-659). Alternative splicing generates additional isoforms of human FBXW7 that have deletions and/or substitutions of the N-terminal 167 amino acids. Within aa 377-707, human FBXW7 shares 99.7% and 88% aa sequence identity with mouse and rat FBXW7, respectively.
  • Long Name:
    F-box and WD Repeat Domain Containing 7
  • Entrez Gene IDs:
    55294 (Human); 50754 (Mouse)
  • Alternate Names:
    AGO; AGOFBXO30; Archipelago Homolog; archipelago, Drosophila, homolog of; Cdc4; CDC4FLJ16457; F-box and WD repeat domain containing 7; F-box and WD-40 domain protein 7 (archipelago homolog, Drosophila); F-box and WD-40 domain-containing protein 7; F-box protein FBW7; F-box protein FBX30; F-box protein SEL-10; F-box/WD repeat-containing protein 7; FBW6; Fbw7; FBW7hCdc4; FBX30; FBX30FLJ11071; FBXO30; FBXW6; FBXW7; hAgo; homolog of C elegans sel-10; SCF(Fbw7); SEL10; SEL-10DKFZp686F23254; SEL10FBW6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Inactivation of EWS reduces PGC-1alpha protein stability and mitochondrial homeostasis.
    Authors: Park J, Kang H, Lee Y, Kang H, Kim J, Chung J, Chang J, McPherron A, Lee S
    Proc Natl Acad Sci U S A, 2015;112(19):6074-9.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse IgG Antibody

WB AF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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