Human Fc gamma  RII/CD32 Antibody

(7 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Fc gamma RII/CD32 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) Fc gamma RIIA is observed, and approximately 10% cross-reactivity with recombinant mouse Fc gamma RIIB is observed, and less than 1% cross-reactivity with rhFc gamma RIIIA is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Fc gamma RII/CD32
    Ala46-Pro217
    Accession # P31994
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Fc gamma  RIIB/C (CD32b/c) (Catalog # 1875-CD)
  • Flow Cytometry
    2.5 µg/106 cells
    Human peripheral blood cells
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 2-6 µg/mL of this antibody will block 50% of the binding of 2 μg/mL of human IgG to immobilized Recombinant Human Fc gamma RIIB/C (CD32b/c) (Catalog # 1875-CD) coated at 2 µg/mL (100 µL/well). At 100 μg/mL, this antibody will block >90% of the binding.
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Fc gamma  RII/CD32 in Human PBMCs. Fc gamma  RII/CD32 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human Fc gamma  RII/CD32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1330) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fc gamma RII/CD32

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10-8‑10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6‑10-7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, FcR gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIB, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5).

Three distinct genes encode the human CD32 group, and the protein products are designated Fc gamma  RIIA, B, and C (1). These receptors are glycoproteins of approximately 40 kDa having two extracellular Ig-like domains. The Fc gamma  RII proteins share 94‑99% amino acid identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. Fc gamma  RIIA associates with FcR gamma, and delivers an activating signal upon ligand binding (3, 5). In contrast, Fc gamma  RIIB delivers an inhibitory signal. Fc gamma  RIIC represents an unequal cross-over event between the IIA and IIB genes. Its extracellular domain shares 99% amino acid identity with Fc gamma  RIIB, but a portion of the cytoplasmic domain is closely related to Fc gamma  RIIA. Fc gamma  RII proteins are expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin.

  • References:
    1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215. 
    2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181. 
    3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
    4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
    5. Ravetch, J. and L. Lanier (2000) Science 290:84.
  • Long Name:
    Fc gamma Receptor II
  • Entrez Gene IDs:
    2212 (Human); 14130 (Mouse)
  • Alternate Names:
    CD32 antigen; CD32B; CD32Fc fragment of IgG, low affinity II, receptor for (CD32); CDw32; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); Fc gamma RII; Fc gamma RIIb; FCG2; Fc-gamma RII-b; fc-gamma-RIIb; FCGR2; FcgRII; fcRII-b; IGFR2; IgG Fc receptor II-b; low affinity immunoglobulin gamma Fc region receptor II-b
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. C-reactive protein induces G2/M phase cell cycle arrest and apoptosis in monocytes through the upregulation of B-cell translocation gene 2 expression.
    Authors: Kim Y, Ryu J, Ryu M, Lim S, Han K, Lim I, Han K
    FEBS Lett, 2014;588(4):625-31.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Detection of serotype-specific antibodies to the four dengue viruses using an immune complex binding (ICB) ELISA.
    Authors: Emmerich, Petra, Mika, Angela, Schmitz, Herbert
    PLoS Negl Trop Dis, 2013;7(12):e2580.
    Species: Human
    Sample Type: Protein
    Application: WB
  3. Self double-stranded (ds)DNA induces IL-1beta production from human monocytes by activating NLRP3 inflammasome in the presence of anti-dsDNA antibodies.
    Authors: Shin M, Kang Y, Lee N, Wahl E, Kim S, Kang K, Lazova R, Kang I
    J Immunol, 2013;190(4):1407-15.
    Species: Human
    Sample Type: Whole Cells
    Application: Blocking
  4. Compromised NK cell-mediated antibody-dependent cellular cytotoxicity in chronic SIV/SHIV infection.
    Authors: He X, Li D, Luo Z, Liang H, Peng H, Zhao Y, Wang N, Liu D, Qin C, Wei Q, Yan H, Shao Y
    PLoS ONE, 2013;8(2):e56309.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Cells
    Application: Neut
  5. C-reactive protein promotes diabetic kidney disease in a mouse model of type 1 diabetes.
    Authors: Liu F, Chen HY, Huang XR, Chung AC, Zhou L, Fu P, Szalai AJ, Lan HY
    Diabetologia, 2011;54(10):2713-23.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. C-reactive protein (CRP) induces chemokine secretion via CD11b/ICAM-1 interaction in human adherent monocytes.
    Authors: Montecucco F, Steffens S, Burger F, Pelli G, Monaco C, Mach F
    J. Leukoc. Biol., 2008;84(4):1109-19.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  7. C-reactive protein induces endothelial cell apoptosis and matrix metalloproteinase-9 production in human mononuclear cells: Implications for the destabilization of atherosclerotic plaque.
    Authors: Nabata A, Kuroki M, Ueba H, Hashimoto S, Umemoto T, Wada H, Yasu T, Saito M, Momomura S, Kawakami M
    Atherosclerosis, 2007;196(1):129-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
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