Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA Capture (Matched Antibody Pair)

Cited:

Immunohistochemistry-Paraffin, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 190710
View all Fc gamma RIIB/C (CD32b/c) Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIB/C
Ala46-Pro217
Accession # P31994

Specificity

Detects human Fc gamma RIIB/C in ELISAs. In a sandwich ELISA shows approximately 40% cross-reactivity with recombinant human (rh) Fc gamma  RIIA and no cross‑reactivity with rhFc gamma RI, rmFc gamma RI, rmFc gamma  RIIB, rhFc gamma RIIIB, rmFc gamma RIII, or rmFcrl3/CD16-2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human Fc gamma  RIIB/C (CD32b/c) Antibody

Human Fc gamma RIIB/CD32b Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human Fc gamma  RIIB/C (CD32b/c) Biotinylated Antibody (Catalog # BAM1875)
  • Standard: Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein, CF (Catalog # 1875-CD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RIIB/C (CD32b/c)

Fc gamma RII, also known as CD32, is a group of three closely related proteins (Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIC) that share greater than 94% amino acid identity in their extracellular domains with Fc gamma RIIB and Fc gamma RIIC having identical extracellular domains. They function as transmembrane receptors for the Fc portion of IgG molecules. These proteins are expressed by various hematopoietic cells including monocytes, macrophages, neutrophils, NK, T and B cells. The Fc gamma RII proteins are involved in phagocytosis of immune complexes and modulation of antibody production by B cells.

Long Name

Fc gamma Receptor IIB/C

Alternate Names

CD32;CD32B;FCG2;FcgammaRIIb;FCGR2;FCGR2B;FCGR2C;FcGRIIB;FcRII-c;IGFR2;Low affinity immunoglobulin gamma Fc region receptor II-b

Entrez Gene IDs

2213 (Human); 14130 (Mouse)

Gene Symbol

FCGR2B

UniProt

P31994

Additional Fc gamma RIIB/C (CD32b/c) Products

Product Documents for Human Fc gamma  RIIB/C (CD32b/c) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Fc gamma  RIIB/C (CD32b/c) Antibody

For research use only

Citations for Human Fc gamma  RIIB/C (CD32b/c) Antibody

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