Detects human FGF-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) FGF-8b and recombinant mouse FGF-8c is observed and less than 2% cross-reactivity with rhFGF acidic, rhFGF basic, rhFGF‑4, rhFGF‑5, rhFGF‑6, rhFGF‑7, rhFGF‑10, and rhFGF‑18 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant human FGF-17 Thr23-Thr216 Accession # O60258
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Measured by its ability to neutralize FGF‑17-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 6-24 µg/mL in the presence of 100 ng/mL Recombinant Human FGF‑17 and 1 µg/mL heparin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Cell Proliferation Induced by FGF‑17 and Neutralization by Human FGF‑17 Antibody. Recombinant Human FGF‑17 (Catalog # 319-FG) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑17 (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF319). The ND50 is typically 6-24 µg/mL in the presence of heparin (1 µg/mL).
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Fibroblast growth factors (FGFs) play multiple biological functions including angiogenesis, mitogenesis, cellular differentiation and wound repairing. All members of the FGF family have a conserved approximately 120 amino acid core with 30‑70% identity. Among FGF family members, FGF-17 is most similar to FGF-8 (60% sequence identity) and FGF-18 (50% sequence identity). The mRNA of FGF-17 was found in midgestation of embryo and multiple adult tissues, and is preferentially expressed in specific sites, such as embryonic brain, developing skeleton and arteries. Human FGF‑17 shares 98.6% amino acid (aa) sequence identity with mouse FGF‑17. Rat FGF‑17 shares 100% aa sequence identity with mouse FGF‑17.
Hoshikawa, M. et al. (1998) Biochem. Biophys. Res. Commun. 244:187.
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