Fibroblast growth factors (FGFs) play multiple biological functions including angiogenesis, mitogenesis, cellular differentiation and wound repairing. All members of the FGF family have a conserved approximately 120 amino acid core with 30‑70% identity. Among FGF family members, FGF-17 is most similar to FGF-8 (60% sequence identity) and FGF-18 (50% sequence identity). The mRNA of FGF-17 was found in midgestation of embryo and multiple adult tissues, and is preferentially expressed in specific sites, such as embryonic brain, developing skeleton and arteries. Human FGF‑17 shares 98.6% amino acid (aa) sequence identity with mouse FGF‑17. Rat FGF‑17 shares 100% aa sequence identity with mouse FGF‑17.
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human FGF-17
Thr23-Thr216
Accession # O60258
Thr23-Thr216
Accession # O60258
Specificity
Detects human FGF-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) FGF-8b and recombinant mouse FGF-8c is observed and less than 2% cross-reactivity with rhFGF acidic, rhFGF basic, rhFGF‑4, rhFGF‑5, rhFGF‑6, rhFGF‑7, rhFGF‑10, and rhFGF‑18 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human FGF‑17 Antibody
Cell Proliferation Induced by FGF‑17 and Neutralization by Human FGF‑17 Antibody.
Recombinant Human FGF‑17 (Catalog # 319-FG) stimulates proliferation in the the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF‑17 (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF319). The ND50 is typically 6-24 µg/mL in the presence of heparin (1 µg/mL).
Applications for Human FGF‑17 Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human FGF‑17 (Catalog # 319-FG)
Sample: Recombinant Human FGF‑17 (Catalog # 319-FG)
Neutralization
Measured by its ability to neutralize FGF‑17-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND50) is typically 6-24 µg/mL in the presence of 100 ng/mL Recombinant Human FGF‑17 and 1 µg/mL heparin.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: FGF-17
References
- Hoshikawa, M. et al. (1998) Biochem. Biophys. Res. Commun. 244:187.
- Xu, J. et al. (1999) Mech. Dev. 83:165.
Long Name
Fibroblast Growth Factor 17
Alternate Names
FGF17
Gene Symbol
FGF17
UniProt
Additional FGF-17 Products
Product Documents for Human FGF‑17 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human FGF‑17 Antibody
For research use only
Related Research Areas
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Protocols
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