Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 349331
View all Fgr Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Fgr
Met1-Phe82
Accession # P09769

Specificity

Detects human Fgr in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Fgr Antibody

Fgr antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

Fgr in HepG2 Human Cell Line.

Fgr was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Fgr Monoclonal Antibody (Catalog # MAB32071) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Fgr Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Daudi human Burkitt's lymphoma cell line and immersion fixed HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Fgr

Fgr is the cellular homolog of the oncogenic protein encoded by the Gardner-Rasheed feline sarcoma virus. A member of the Src family of nonreceptor tyrosine kinases, Fgr is predominantly expressed in neutrophils, macrophages, monocytes, and natural killer cells. Mice lacking Fgr fail to recruit eosinophils to the lung during allergic airway inflammation.

Long Name

Gardner-Rasheed Feline Sarcoma Viral Oncogene Homolog

Alternate Names

Src2

Entrez Gene IDs

2268 (Human); 14191 (Mouse); 79113 (Rat)

Gene Symbol

FGR

UniProt

P09769

Additional Fgr Products

Product Documents for Human Fgr Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Fgr Antibody

For research use only

Related Research Areas

Intracellular Kinases

Customer Reviews for Human Fgr Antibody

There are currently no reviews for this product. Be the first to review Human Fgr Antibody and earn rewards!

Have you used Human Fgr Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies