Human FoxJ1 Antibody

Detection of Human FoxJ1 by Simple Western^TM.

Simple Western lane view shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and T47D human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for FoxJ1 at approximately 58 kDa (as indicated) using 10 µg/mL of Goat Anti-Human FoxJ1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3619) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Detection of FoxJ1 by Immunohistochemistry

Heterogenous neural cells in the human developing spinal cord.a, Representative images showing validation of newborn neurons and glial cells in the developing human spinal cord by HybISS. Scale bar, 200 μm. b, Representative confocal images showing immunostaining of newborn astrocytes (b) and ependymal cells (c) at W5, whereas OPCs are not born at W5 yet (d). Two independent experiments for each panel (a–d) were performed. Scale bars, 200 μm and 50 μm for low and high magnification, respectively. Rectangles indicate enlarged areas. e, UMAP illustrating the heterogenous cell types or cell states of different neural cell populations. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37095395), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of FoxJ1 by Immunocytochemistry/ Immunofluorescence

SARS-CoV-2 provokes senescence-like phenotype in hBOs.a, RT-qPCR analysis of ACE2 gene expression in HDFs, HepG2, and human bronchial organoid (hBO). b, Schematic diagram of the genome (genomic-CoV2) and subgenomic (subgenomic-CoV2) RNAs and the positions of the RT-qPCR primers used (upper panel). hBOs were infected with SARS-CoV-2 at MOI of 0.1 for the indicated periods (lower panel). These cells were analyzed by RT-qPCR to determine the genomic and subgenomic SARS-CoV-2 RNAs (lower panel), gene expression of virus-induced cytokines and SASP factors (c), or by immunofluorescence analysis of KRT5 (basal cell marker) expression [red], p16INK4a expression [green], and DAPI [blue] (d), FoxJ1 (ciliated cell marker) expression [red], p16INK4a expression [green], and DAPI [blue] (e) or cleaved caspase-3 (C-casp 3) [red], SARS-CoV-2 spike protein (CoV2-S) [green], and DAPI [blue] (f) in Mock or SARS-CoV-2-infected cells (CoV2) on day 6 after SARS-CoV-2 infection. (Scale bars, 50 μm). The histograms indicate the percentages of p16INK4a expressing cells in KRT5 or FoxJ1 positive cell, and cleaved caspase-3 positive cell percentages in SARS-CoV-2 spike protein negative (CoV2-) or positive (CoV2 + ) cells. For all graphs, error bars indicate mean ± standard deviation (s.d.) of biological triplicate. Statistical significance was determined with two-tailed unpaired Student’s t test in (a), (d) and (e), and with two-way ANOVA followed by sidak’s multiple comparison test in (b), (c), and (f).Source data Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37117754), licensed under a CC-BY license. Not internally tested by R&D Systems.