Human Frizzled-5 Antibody

Catalog # Availability Size / Price Qty
AF1617
AF1617-SP
Product Details
Citations (8)
FAQs
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Human Frizzled-5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Frizzled-5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Frizzled-1, rmFrizzled-2, rmFrizzled-3, rmFrizzled-6, rmFrizzled-7, and rmFrizzled-8 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Frizzled‑5
Ala27-Pro167
Accession # AAC50385
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Frizzled‑5 Fc Chimera (Catalog # 1617-FZ)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-5

Wnt signaling is involved in variety of developmental processes including cell fate determination, cell polarity, tissue patterning and control of cell proliferation. Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway. The founding member of this family was identified in Drosophila based on its role in tissue polarity in the adult cuticle and named for the disorganized appearance of bristle hairs on the mutant. The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain, a variable-length linker region, a seven-pass transmembrane domain, and a variable-length C-terminal tail. One of the most conserved regions of the Frizzled (Frz) proteins is the extracellular cysteine-rich domain (CRD) which spans approximately 120 amino acid (aa) and contains 10 invariant cysteines (1). Human Frz-5 shows 95% aa identity to mouse Frz-5 in the CRD region. In the mouse, Frz-5 is expressed in adult tissues (heart and kidney), as well as embryonic tissues (telencephalon, eye, and lung bud) (2, 3). Null mutations in Frz-5 reveal that it plays a role in the formation and maintenance of the extra-embryonic vasculature (3). Functional interactions with Frz-5 have been reported for Wnt-5a, Wnt-10b, Wnt-2b, and Wnt-7a, implicating these Wnts as ligands for the Frz-5 receptor (3-5).

Two distinct Wnt signal transduction pathways have been characterized. One is the canonical Wnt/ beta -catenin pathway that is involved in diverse biological mechanisms such as dorsal/ventral development in Xenopus embryos and mammalian tumor formation. Frz-5 is implicated in this pathway based on its ability to induce beta -catenin target genes in the presence of ligand (5). However, Frz-5 is also implicated in beta -catenin independent pathways (4, 6).

References
  1. Wang, Y. et al. (1996) J. Biol. Chem 271:4468.
  2. Borello, U. et al. (1999) Mech. Dev. 89:173.
  3. Ishikawa, T. et al. (2001) Development 128:25.
  4. He, X. et al. (1997) Science 275:1652.
  5. Caricasole, A. et al. (2003) J. Biol. Chem 278:37024.
  6. Veeman, M. et al. (2003) Dev. Cell 5:367.
Entrez Gene IDs
7855 (Human); 14367 (Mouse)
Alternate Names
C2orf31; chromosome 2 open reading frame 31; DKFZP434E2135; frizzled (Drosophila) homolog 5; frizzled homolog 5 (Drosophila); Frizzled5; Frizzled-5; Frz-5; FZ-5; FZD5; fzE5; HFZ5; MGC129692; seven-transmembrane receptor frizzled-5; Wnt receptor

Product Datasheets

Citations for Human Frizzled-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Secreted phosphoprotein-1 accelerates the progression of human colorectal cancer through activating &beta-catenin signaling
    Authors: J Yi, Y Liu, L Zhang, C Fang
    Oncology Letters, 2021;21(5):372.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Overexpression of G3BP1 facilitates the progression of colon cancer by activating &beta?catenin signaling
    Authors: Y Li, J Wang, S Zhong, J Li, W Du
    Molecular Medicine Reports, 2020;22(5):4403-4411.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Wnt-5a/Frizzled9 receptor signaling through the G?o/G?? complex regulates dendritic spine formation
    J Biol Chem, 2016;0(0):.
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Bioassay
  4. Wnts enhance neurotrophin-induced neuronal differentiation in adult bone-marrow-derived mesenchymal stem cells via canonical and noncanonical signaling pathways.
    Authors: Tsai H, Deng W, Lai W, Chiu W, Yang C, Tsai Y, Hwang S, Renshaw P
    PLoS ONE, 2014;9(8):e104937.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  5. WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins.
    Authors: Kilander MB, Dijksterhuis JP, Ganji RS, Bryja V, Schulte G
    Cell. Signal., 2011;23(3):550-4.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. WNT5A Signaling Contributes to Abeta-Induced Neuroinflammation and Neurotoxicity.
    Authors: Li B, Zhong L, Yang X, Andersson T, Huang M, Tang SJ
    PLoS ONE, 2011;6(8):e22920.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Evidence for altered Wnt signaling in psoriatic skin.
    Authors: Gudjonsson JE, Johnston A, Stoll SW
    J. Invest. Dermatol., 2010;130(7):1849-59.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Amyloid-beta binds to the extracellular cysteine-rich domain of Frizzled and inhibits Wnt/beta-catenin signaling.
    Authors: Magdesian MH, Carvalho MM, Mendes FA, Saraiva LM, Juliano MA, Juliano L, Garcia-Abreu J, Ferreira ST
    J. Biol. Chem., 2008;283(14):9359-68.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: ELISA Development

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