Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Fish - Danio rerio (Zebrafish)

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Bioassay, ELISA Development, ELISA Development (Capture)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Furin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Furin
Asp108-Glu715
Accession # P09958

Specificity

Detects human Furin in direct ELISAs and Western blots. In direct ELISAs, less than 40% cross-reactivity with recombinant mouse Furin is observed, and less than 1% cross-reactivity with recombinant human (rh) PC-1 and rhPC-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Furin Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Furin (Catalog # 1503-SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Furin (Catalog # 1503-SE)

Reviewed Applications

Read 1 review rated 5 using AF1503 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Furin

Furin is a member of the proprotein convertase (PC) family, which belongs to the subtilisin superfamily of serine protease (1-3). As a cellular protease, Furin processes a variety of proproteins in secretory pathway compartments by cleaving after Arg-Xaa-Lys/Arg-Arg-like motifs, which usually reside at the end of the pro regions of these proproteins. Examples of the proprotein substrates are growth factors and receptors, extracellular matrix proteins, and other proteases. Furin has an essential role in embryogenesis and homeostasis and is implicated in various pathologies such as cancer, neurodegenerative diseases and anthrax. It is synthesized as a 794 amino acid type I transmembrane protein precursor with a signal peptide (residues 1-24), a pro region (residues 25-107), which play a crucial role in the folding, activation and transport of Furin, and a mature chain (residues 108-794) (1-3). The mature chain consists of the subtilisin-like catalytic domain, a P domain, which is essential for enzyme activity and the modulation of pH and calcium requirements, and a cytoplasmic domain, which controls the localization and sorting of Furin in the trans-Golgi network/endosomal system. The purified recombinant human Furin (residues 108-715) corresponds to the mature enzyme terminated before the transmembrane domain.

References

  1. Van den Ouweland, A.M. et al. (1990) Nucleic Acids Res. 18:664.
  2. Barr, P.J. et al. (1991) DNA Cell Biol. 10:319.
  3. Thomas, G. (2002) Nature Rev. Mol. Cell Biol. 3:753.

Alternate Names

FUR, PACE, PCSK3, SPC1

Entrez Gene IDs

5045 (Human); 18550 (Mouse)

Gene Symbol

FURIN

UniProt

P09958

Additional Furin Products

Product Documents for Human Furin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Furin Antibody

For research use only

Citations for Human Furin Antibody

Customer Reviews for Human Furin Antibody (1)

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  • Human Furin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: A549 human lung carcinoma cell line
    Species: Human
    Verified Customer | Posted 10/26/2021
    Human Furin Antibody AF1503

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