Detects human Galectin-14 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Galectin-1, -2, -3, -4, -7, -8, -10, or recombinant mouse Galectin-9 is observed.
Monoclonal Mouse IgG2B Clone # 511124
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Galectin-14 Met1-Asp139 Accession # Q8TCE9
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Galectin‑14 by Western Blot. Western blot shows lysates of human eosinophil. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Galectin‑14 Monoclonal Antibody (Catalog # MAB5744) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Galectin‑14 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Galectin-14, also known as placental protein 13-like (PPL13), belongs the galectin family of carbohydrate binding proteins. It is expressed intracellularly in placenta and eosinophils and is released by eosinophils following allergen stimulation. Galectin-14 may be involved in the development of allergic inflammation.
Entrez Gene IDs:
Charcot-Leyden crystal protein 2; CLC2; CLC2placental protein 13-like protein; Gal-14; Galectin14; galectin-14; lectin, galactoside-binding, soluble, 14; LGALS14; MGC22235; PPL13; PPL13placental protein 13-like
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