Human Galectin-3BP/MAC-2BP Antibody
Human Galectin-3BP/MAC-2BP Antibody Summary
15% cross‑reactivity with recombinant mouse MAC‑2BP is observed.
Val19-Asp585
Accession # Q08380
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
View Larger
Detection of Human
Galectin‑3BP/MAC‑2BP by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin‑3BP/MAC‑2BP at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
View Larger
Galectin‑3BP/MAC‑2BP in HeLa Human Cell Line. Galectin‑3BP/MAC‑2BP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
View Larger
Detection of Human
Galectin‑3BP/MAC‑2BP by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑3BP/MAC‑2BP at approximately 115 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-3BP/MAC-2BP
Galectin-3 binding protein (Galectin-3BP), also known as MAC-2 binding protein (MAC-2BP or M2BP), and the 90 kDa tumor associated antigen (TAA90K or 90K), is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily (1, 2). Galectin-3BP binds Galectin-3 (formerly MAC-2) with high affinity, but also binds Galectins -1 and -7, several collagen types, fibronectin, beta 1 integrins and nidogen (3, 6, 7). It is widely expressed in all extracellular fluids and in pericellular areas of cell-rich tissues (1-3). The 585 amino acid (aa) human Galectin-3BP contains an 18 aa signal sequence and four definitive domains (4-6). Domain 1 is a group A scavenger receptor domain (4), domain 2 is a BTB/POZ domain that strongly mediates dimerization (5), and domain 3 is an IVR domain, that is also found following the POZ domain in Drosophila kelch protein. Although little is known about domain 4, recombinant domains 3 and 4 reproduce the solid-phase adhesion profile of full-length Galectin-3BP (5, 6). Glycosylation at seven N-linked sites, generates a molecular size of 85-97 kDa (1, 2, 6). Galectin-3BP dimers form linear and ring-shaped oligomers, most commonly decamers and dodecamers (3, 5). In vitro, Galectin-3BP has been shown to stimulate natural killer cells and lymphokine-activated killer cell activity (2). High Galectin-3BP expression has been correlated with tumor aggressiveness in several, but not all, study systems (7). Mature human Galectin-3BP shares 69% aa identity with mouse cyclophilin C-associated protein (CyCAP), which does not appear to bind Galectin-3 (8). Human Galectin-3BP also shares 73%, 67% and 68% aa identity with relatively uncharacterized orthologs in dog, rat and cow, respectively. A human N-terminally truncated sequence that begins within the BTB/POZ domain (aa 196) has been reported.
- Koths, K. et al. (1993) J. Biol. Chem. 268:14245.
- Ullrich, A. et al. (1994) J. Biol. Chem. 269:18401.
- Sasaki, T. et al. (1998) EMBO J. 17:1606.
- Hohenester, E. et al. (1999) Nat. Struct. Biol. 6:228.
- Muller, S. A. et al. (1999) J. Mol. Biol. 291:801.
- Hellstern, S. et al. (2002) J. Biol. Chem. 277:15690.
- Grassadonia, A. et al. (2004) Glycoconj. J. 19:551.
- Jalkanen, K. et al. (2001) Eur. J. Immunol. 31:3075.
Product Datasheets
Citations for Human Galectin-3BP/MAC-2BP Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
7
Citations: Showing 1 - 7
Filter your results:
Filter by:
-
Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques
Authors: SR Langley, K Willeit, A Didangelos, LP Matic, P Skroblin, J Barallobre, M Lengquist, G Rungger, A Kapustin, L Kedenko, C Molenaar, R Lu, T Barwari, G Suna, X Yin, B Iglseder, B Paulweber, P Willeit, J Shalhoub, G Pasterkamp, AH Davies, C Monaco, U Hedin, CM Shanahan, J Willeit, S Kiechl, M Mayr
J. Clin. Invest, 2017;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Lectin, galactoside-binding soluble 3 binding protein promotes 17-N-Allylamino-17-demethoxygeldanamycin resistance through PI3K/Akt pathway in lung cancer cell line
Authors: JK Woo, JE Jang, JH Kang, JK Seong, YS Yoon, HC Kim, SJ Lee, SH Oh
Mol. Cancer Ther, 2017;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
M2BP inhibits HIV-1 virion production in a vimentin filaments-dependent manner
Sci Rep, 2016;6(0):32736.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Human galectin 3 binding protein interacts with recombinant adeno-associated virus type 6.
J. Virol., 2012;86(12):6620-31.
Species: Human
Sample Types: Serum
Applications: Western Blot -
A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma.
Authors: Silverman A, Nakata R, Shimada H, Sposto R, DeClerck Y
Cancer Res, 2012;72(9):2228-38.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31.
Authors: Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H
Exp. Cell Res., 2011;317(7):976-93.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stroma.
Authors: Fukaya Y, Shimada H, Wang LC, Zandi E, DeClerck YA
J. Biol. Chem., 2008;283(27):18573-81.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsCell and Tissue Staining Kits
Isotype Controls
Reconstitution Buffers
Secondary Antibodies
Reviews for Human Galectin-3BP/MAC-2BP Antibody
Average Rating: 3.7 (Based on 3 Reviews)
Have you used Human Galectin-3BP/MAC-2BP Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
