Human Galectin-3BP/MAC-2BP Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Galectin‑3BP/MAC‑2BP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately
    15% cross‑reactivity with recombinant mouse MAC‑2BP is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Galectin‑3BP/MAC‑2BP
    Val19-Asp585
    Accession # Q08380
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunocytochemistry
    1-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human
Galectin‑3BP/MAC‑2BP by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin‑3BP/MAC‑2BP at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
Galectin‑3BP/MAC‑2BP in HeLa Human Cell Line. Galectin‑3BP/MAC‑2BP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human
Galectin‑3BP/MAC‑2BP by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑3BP/MAC‑2BP at approximately 115 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.        
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-3BP/MAC-2BP

Galectin-3 binding protein (Galectin-3BP), also known as MAC-2 binding protein (MAC-2BP or M2BP), and the 90 kDa tumor associated antigen (TAA90K or 90K), is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily (1, 2). Galectin-3BP binds Galectin-3 (formerly MAC-2) with high affinity, but also binds Galectins -1 and -7, several collagen types, fibronectin, beta 1 integrins and nidogen (3, 6, 7). It is widely expressed in all extracellular fluids and in pericellular areas of cell-rich tissues (1-3). The 585 amino acid (aa) human Galectin-3BP contains an 18 aa signal sequence and four definitive domains (4-6). Domain 1 is a group A scavenger receptor domain (4), domain 2 is a BTB/POZ domain that strongly mediates dimerization (5), and domain 3 is an IVR domain, that is also found following the POZ domain in Drosophila kelch protein. Although little is known about domain 4, recombinant domains 3 and 4 reproduce the solid-phase adhesion profile of full-length Galectin-3BP (5, 6). Glycosylation at seven N-linked sites, generates a molecular size of 85-97 kDa (1, 2, 6). Galectin-3BP dimers form linear and ring-shaped oligomers, most commonly decamers and dodecamers (3, 5). In vitro, Galectin-3BP has been shown to stimulate natural killer cells and lymphokine-activated killer cell activity (2). High Galectin-3BP expression has been correlated with tumor aggressiveness in several, but not all, study systems (7). Mature human Galectin-3BP shares 69% aa identity with mouse cyclophilin C-associated protein (CyCAP), which does not appear to bind Galectin-3 (8). Human Galectin-3BP also shares 73%, 67% and 68% aa identity with relatively uncharacterized orthologs in dog, rat and cow, respectively. A human N-terminally truncated sequence that begins within the BTB/POZ domain (aa 196) has been reported.

  • References:
    1. Koths, K. et al. (1993) J. Biol. Chem. 268:14245.
    2. Ullrich, A. et al. (1994) J. Biol. Chem. 269:18401.
    3. Sasaki, T. et al. (1998) EMBO J. 17:1606.
    4. Hohenester, E. et al. (1999) Nat. Struct. Biol. 6:228.
    5. Muller, S. A. et al. (1999) J. Mol. Biol. 291:801.
    6. Hellstern, S. et al. (2002) J. Biol. Chem. 277:15690.
    7. Grassadonia, A. et al. (2004) Glycoconj. J. 19:551.
    8. Jalkanen, K. et al. (2001) Eur. J. Immunol. 31:3075.
  • Long Name:
    Galectin-3 Binding Protein
  • Entrez Gene IDs:
    3959 (Human)
  • Alternate Names:
    90K; Basement membrane autoantigen p105; BTBD17B; galectin-3-binding protein; Galectin3BP; Galectin-3BP; L3 antigen; Lectin galactoside-binding soluble 3-binding protein; lectin, galactoside-binding, soluble, 3 binding protein; LGALS3BP; M2BP; Mac-2 BP; Mac-2-binding protein; MAC2BP; MAC-2BP; MAC-2-BP; serum protein 90K; Tumor-associated antigen 90K
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Lectin, galactoside-binding soluble 3 binding protein promotes 17-N-Allylamino-17-demethoxygeldanamycin resistance through PI3K/Akt pathway in lung cancer cell line
    Authors: JK Woo, JE Jang, JH Kang, JK Seong, YS Yoon, HC Kim, SJ Lee, SH Oh
    Mol. Cancer Ther, 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. M2BP inhibits HIV-1 virion production in a vimentin filaments-dependent manner
    Sci Rep, 2016;6(0):32736.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma.
    Authors: Silverman A, Nakata R, Shimada H, Sposto R, DeClerck Y
    Cancer Res, 2012;72(9):2228-38.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Human galectin 3 binding protein interacts with recombinant adeno-associated virus type 6.
    J. Virol., 2012;86(12):6620-31.
    Species: Human
    Sample Type: Serum
    Application: WB
  5. Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31.
    Authors: Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H
    Exp. Cell Res., 2011;317(7):976-93.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  6. Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stroma.
    Authors: Fukaya Y, Shimada H, Wang LC, Zandi E, DeClerck YA
    J. Biol. Chem., 2008;283(27):18573-81.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: WB
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