Galectin-3 binding protein (Galectin-3BP), also known as MAC-2 binding protein (MAC-2BP or M2BP), and the 90 kDa tumor associated antigen (TAA90K or 90K), is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily (1, 2). Galectin-3BP binds Galectin-3 (formerly MAC-2) with high affinity, but also binds Galectins -1 and -7, several collagen types, fibronectin, beta 1 integrins and nidogen (3, 6, 7). It is widely expressed in all extracellular fluids and in pericellular areas of cell-rich tissues (1-3). The 585 amino acid (aa) human Galectin-3BP contains an 18 aa signal sequence and four definitive domains (4-6). Domain 1 is a group A scavenger receptor domain (4), domain 2 is a BTB/POZ domain that strongly mediates dimerization (5), and domain 3 is an IVR domain, that is also found following the POZ domain in Drosophila kelch protein. Although little is known about domain 4, recombinant domains 3 and 4 reproduce the solid-phase adhesion profile of full-length Galectin-3BP (5, 6). Glycosylation at seven N-linked sites, generates a molecular size of 85-97 kDa (1, 2, 6). Galectin-3BP dimers form linear and ring-shaped oligomers, most commonly decamers and dodecamers (3, 5). In vitro, Galectin-3BP has been shown to stimulate natural killer cells and lymphokine-activated killer cell activity (2). High Galectin-3BP expression has been correlated with tumor aggressiveness in several, but not all, study systems (7). Mature human Galectin-3BP shares 69% aa identity with mouse cyclophilin C-associated protein (CyCAP), which does not appear to bind Galectin-3 (8). Human Galectin-3BP also shares 73%, 67% and 68% aa identity with relatively uncharacterized orthologs in dog, rat and cow, respectively. A human N-terminally truncated sequence that begins within the BTB/POZ domain (aa 196) has been reported.
Human Galectin‑3BP/MAC‑2BP Antibody
R&D Systems | Catalog # AF2226
Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Val19-Asp585
Accession # Q08380
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human Galectin‑3BP/MAC‑2BP Antibody
Detection of Human Galectin‑3BP/MAC‑2BP by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1.0 µg/mL of Goat Anti-Human Galectin-3BP/MAC-2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Galectin-3BP/MAC-2BP at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Galectin‑3BP/MAC‑2BP in HeLa Human Cell Line.
Galectin-3BP/MAC-2BP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Galectin-3BP/MAC-2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red;NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Galectin‑3BP/MAC‑2BP by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-3BP/MAC-2BP at approximately 115 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin-3BP/MAC-2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Galectin-3BP/MAC-2BP by Western Blot
GAL3BP-induced inhibition of A beta production.a, the amounts of A beta 40 and A beta 42 in the conditioned media from H4-APPsw cells (Cont.) and H4-APPsw cells overexpressing GAL3BP (+GAL3BP) were measured using ELISAs. b, conditioned media were prepared from control HEK293 cells (Cont., lane 1), HEK293 cells with GAL3BP knockdown via RNAi (+RNAi, lane 2), and HEK293 cells with GAL3BP knockdown and GPLD1 overexpression (+RNAi, +GPLD1, lane 3) and subsequently added to H4-APPsw cells to test for their suppressive activity on A beta production. Western blot analysis confirmed the efficiency of RNAi for the amount of GAL3BP expression in the culture media (bottom). c, recombinant GAL3BP (5 μg/ml) prepared from HEK293 cells was added to H4-APPsw cell cultures, and the amounts of A beta 40 and A beta 42 in the media were determined. d, commercially available recombinant GAL3BP was added to H4-APPsw cell culture, and the amounts of A beta 40 and A beta 42 in the media were measured. a–d, the values in this graph are presented relative to the corresponding controls (n = 3, mean ± S.D. (error bars)). If error bars representing S.D. values are not visible, they are too small to be displayed. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus control by one-way ANOVA with Tukey's post-hoc test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31996371), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human Galectin‑3BP/MAC‑2BP Antibody
Immunocytochemistry
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Simple Western
Sample: HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Western Blot
Sample: HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line
Reviewed Applications
Read 6 reviews rated 4 using AF2226 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: Galectin-3BP/MAC-2BP
References
- Koths, K. et al. (1993) J. Biol. Chem. 268:14245.
- Ullrich, A. et al. (1994) J. Biol. Chem. 269:18401.
- Sasaki, T. et al. (1998) EMBO J. 17:1606.
- Hohenester, E. et al. (1999) Nat. Struct. Biol. 6:228.
- Muller, S. A. et al. (1999) J. Mol. Biol. 291:801.
- Hellstern, S. et al. (2002) J. Biol. Chem. 277:15690.
- Grassadonia, A. et al. (2004) Glycoconj. J. 19:551.
- Jalkanen, K. et al. (2001) Eur. J. Immunol. 31:3075.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional Galectin-3BP/MAC-2BP Products
Product Documents for Human Galectin‑3BP/MAC‑2BP Antibody
Certificate of Analysis
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Product Specific Notices for Human Galectin‑3BP/MAC‑2BP Antibody
For research use only
Related Research Areas
Citations for Human Galectin‑3BP/MAC‑2BP Antibody
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Customer Images
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 01/14/2021
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Application: ELISASample Tested: PlasmaSpecies: HumanVerified Customer | Posted 11/10/2018
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Application: MicroarraysSample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 11/07/2018
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Application: MicroarraySample Tested: EDTA PlasmaSpecies: HumanVerified Customer | Posted 10/31/2018
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Application: Western BlotSample Tested: See PMID 22496229Species: HumanVerified Customer | Posted 01/06/2015
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Application: Western BlotSample Tested: See PMID 21182835Species: HumanVerified Customer | Posted 01/06/2015
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars