Human Galectin-3BP/MAC-2BP Antibody

Name: Human Galectin-3BP/MAC-2BP Antibody
Brand: R&D Systems
SKU: AF2226-SP
Rating: 3.7 (3 reviews)

AF2226 7 Citations 3 Reviews Datasheet / COA / SDS

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Human Galectin-3BP/MAC-2BP Antibody in Western Blot

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Citations (7)

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Reviews (3)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Galectin-3BP/MAC-2BP Antibody Summary

Species Reactivity

Human

Specificity

Detects human Galectin‑3BP/MAC‑2BP in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Galectin-1, rhGalectin-2, rhGalectin-7, rhGalectin-8, rhGalectin-9, and recombinant mouse (rm) Galectin-1 is observed. In direct ELISAs, approximately 1% cross-reactivity with rmGalectin-9, approximately 8% cross-reactivity with rmGalectin-3, and approximately 15% cross‑reactivity with rmMAC‑2BP is observed. In Western blots, approximately 15% cross‑reactivity with recombinant mouse MAC‑2BP is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Galectin‑3BP/MAC‑2BP
Val19-Asp585
Accession # Q08380

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.2 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunocytochemistry

1-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot

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Detection of Human
Galectin‑3BP/MAC‑2BP by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin‑3BP/MAC‑2BP at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Galectin‑3BP/MAC‑2BP in HeLa Human Cell Line. Galectin‑3BP/MAC‑2BP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western

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Detection of Human
Galectin‑3BP/MAC‑2BP by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑3BP/MAC‑2BP at approximately 115 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-3BP/MAC-2BP

Galectin-3 binding protein (Galectin-3BP), also known as MAC-2 binding protein (MAC-2BP or M2BP), and the 90 kDa tumor associated antigen (TAA90K or 90K), is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily (1, 2). Galectin-3BP binds Galectin-3 (formerly MAC-2) with high affinity, but also binds Galectins -1 and -7, several collagen types, fibronectin, beta 1 integrins and nidogen (3, 6, 7). It is widely expressed in all extracellular fluids and in pericellular areas of cell-rich tissues (1-3). The 585 amino acid (aa) human Galectin-3BP contains an 18 aa signal sequence and four definitive domains (4-6). Domain 1 is a group A scavenger receptor domain (4), domain 2 is a BTB/POZ domain that strongly mediates dimerization (5), and domain 3 is an IVR domain, that is also found following the POZ domain in Drosophila kelch protein. Although little is known about domain 4, recombinant domains 3 and 4 reproduce the solid-phase adhesion profile of full-length Galectin-3BP (5, 6). Glycosylation at seven N-linked sites, generates a molecular size of 85-97 kDa (1, 2, 6). Galectin-3BP dimers form linear and ring-shaped oligomers, most commonly decamers and dodecamers (3, 5). In vitro, Galectin-3BP has been shown to stimulate natural killer cells and lymphokine-activated killer cell activity (2). High Galectin-3BP expression has been correlated with tumor aggressiveness in several, but not all, study systems (7). Mature human Galectin-3BP shares 69% aa identity with mouse cyclophilin C-associated protein (CyCAP), which does not appear to bind Galectin-3 (8). Human Galectin-3BP also shares 73%, 67% and 68% aa identity with relatively uncharacterized orthologs in dog, rat and cow, respectively. A human N-terminally truncated sequence that begins within the BTB/POZ domain (aa 196) has been reported.

References

Koths, K. et al. (1993) J. Biol. Chem. 268:14245.
Ullrich, A. et al. (1994) J. Biol. Chem. 269:18401.
Sasaki, T. et al. (1998) EMBO J. 17:1606.
Hohenester, E. et al. (1999) Nat. Struct. Biol. 6:228.
Muller, S. A. et al. (1999) J. Mol. Biol. 291:801.
Hellstern, S. et al. (2002) J. Biol. Chem. 277:15690.
Grassadonia, A. et al. (2004) Glycoconj. J. 19:551.
Jalkanen, K. et al. (2001) Eur. J. Immunol. 31:3075.

Long Name

Galectin-3 Binding Protein

Entrez Gene IDs

3959 (Human)

Alternate Names

90K; Basement membrane autoantigen p105; BTBD17B; galectin-3-binding protein; Galectin3BP; Galectin-3BP; L3 antigen; Lectin galactoside-binding soluble 3-binding protein; lectin, galactoside-binding, soluble, 3 binding protein; LGALS3BP; M2BP; Mac-2 BP; Mac-2-binding protein; MAC2BP; MAC-2BP; MAC-2-BP; serum protein 90K; Tumor-associated antigen 90K

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Galectin-3BP/MAC-2BP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques
Authors: SR Langley, K Willeit, A Didangelos, LP Matic, P Skroblin, J Barallobre, M Lengquist, G Rungger, A Kapustin, L Kedenko, C Molenaar, R Lu, T Barwari, G Suna, X Yin, B Iglseder, B Paulweber, P Willeit, J Shalhoub, G Pasterkamp, AH Davies, C Monaco, U Hedin, CM Shanahan, J Willeit, S Kiechl, M Mayr
J. Clin. Invest, 2017;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Lectin, galactoside-binding soluble 3 binding protein promotes 17-N-Allylamino-17-demethoxygeldanamycin resistance through PI3K/Akt pathway in lung cancer cell line
Authors: JK Woo, JE Jang, JH Kang, JK Seong, YS Yoon, HC Kim, SJ Lee, SH Oh
Mol. Cancer Ther, 2017;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
M2BP inhibits HIV-1 virion production in a vimentin filaments-dependent manner
Sci Rep, 2016;6(0):32736.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Human galectin 3 binding protein interacts with recombinant adeno-associated virus type 6.
J. Virol., 2012;86(12):6620-31.
Species: Human
Sample Types: Serum
Applications: Western Blot
A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma.
Authors: Silverman A, Nakata R, Shimada H, Sposto R, DeClerck Y
Cancer Res, 2012;72(9):2228-38.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31.
Authors: Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H
Exp. Cell Res., 2011;317(7):976-93.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stroma.
Authors: Fukaya Y, Shimada H, Wang LC, Zandi E, DeClerck YA
J. Biol. Chem., 2008;283(27):18573-81.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot