Human Galectin-3BP/MAC-2BP Antibody

• Species Reactivity
  Human
• Specificity
  Detects human Galectin‑3BP/MAC‑2BP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately
  15% cross‑reactivity with recombinant mouse MAC‑2BP is observed.
• Source
  Polyclonal Goat IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  Mouse myeloma cell line NS0-derived recombinant human Galectin‑3BP/MAC‑2BP
  Val19-Asp585
  Accession # Q08380
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Western Blot
  0.2 µg/mL
  See below
• Simple Western
  10 µg/mL
  See below
• Immunocytochemistry
  1-15 µg/mL
  See below

Western Blot click image to view larger	Detection of Human Galectin‑3BP/MAC‑2BP by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin‑3BP/MAC‑2BP at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry click image to view larger	Galectin‑3BP/MAC‑2BP in HeLa Human Cell Line. Galectin‑3BP/MAC‑2BP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Simple Western click image to view larger	Detection of Human Galectin‑3BP/MAC‑2BP by Simple WesternTM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑3BP/MAC‑2BP at approximately 115 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reconstitution
  Reconstitute at 0.2 mg/mL in sterile PBS.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• References:
  1. Koths, K. et al. (1993) J. Biol. Chem. 268:14245.
  2. Ullrich, A. et al. (1994) J. Biol. Chem. 269:18401.
  3. Sasaki, T. et al. (1998) EMBO J. 17:1606.
  4. Hohenester, E. et al. (1999) Nat. Struct. Biol. 6:228.
  5. Muller, S. A. et al. (1999) J. Mol. Biol. 291:801.
  6. Hellstern, S. et al. (2002) J. Biol. Chem. 277:15690.
  7. Grassadonia, A. et al. (2004) Glycoconj. J. 19:551.
  8. Jalkanen, K. et al. (2001) Eur. J. Immunol. 31:3075.

• Long Name:
  Galectin-3 Binding Protein

• Entrez Gene IDs:
  3959 (Human)

• Alternate Names:
  90K; Basement membrane autoantigen p105; BTBD17B; galectin-3-binding protein; Galectin3BP; Galectin-3BP; L3 antigen; Lectin galactoside-binding soluble 3-binding protein; lectin, galactoside-binding, soluble, 3 binding protein; LGALS3BP; M2BP; Mac-2 BP; Mac-2-binding protein; MAC2BP; MAC-2BP; MAC-2-BP; serum protein 90K; Tumor-associated antigen 90K

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