Human GDF-11/GDF-8 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human GDF-11/BMP-11 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with mouse GDF-8 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 743835
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human GDF-11/BMP-11
    Asn299-Ser407
    Accession # O95390
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    Measured by its ability to neutralize GDF‑11/BMP‑11 induction of hemoglobin expression in the K562 human chronic myelogenous leukemia cell line, Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The Neutralization Dose (ND50) is typically 0.04-0.2 μg/mL in the presence of 25 ng/mL Recombinant Human GDF‑11/BMP‑11. This antibody also neutralizes GDF-8 in a similar K562 bioassay.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Induction of Hemoglobin Expression by GDF‑11/BMP‑11 and Neutralization by anti-GDF‑11/GDF‑8 Antibody. Recombinant Human
GDF-11/BMP-11 (Catalog # 1958-GD) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the pseudoperoxidase assay. Induction of hemoglobin expression by GDF‑11/BMP‑11 (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human GDF‑11/GDF‑8 Monoclonal Antibody (Catalog # MAB19582). The ND50 is typically 0.04-0.2 μg/mL. This antibody also neutralizes GDF-8 in a similar K562 bioassay.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GDF-11/GDF-8
Growth Differentiation Factor 11 (GDF-11), also known as BMP-11, is a member of the TGF-beta superfamily and is highly related to GDF-8. GDF-11 encodes a 407 amino acid (aa) prepropeptide processed into a 109 aa mature protein. Mature GDF-11 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members; however, like the TGF-beta s, Activins and GDF-8, GDF-11 also contains one extra pair of cysteine residues. At the amino acid sequence level, mature human, mouse, rat and chicken GDF-11 are 99-100% identical. Mature GDF-11 and GDF-8 share 90% amino acid sequence identity. GDF-11 is expressed in diverse regions of the mouse embryo: tailbud, somitic precursors, limbs, mandibular and branchial arches, dorsal neural tube, odontoblasts, nasal epithelium, and particular regions of the brain (1). GDF-11 signals through the Activin type II receptors and induces phosphorylation of Smad2 to mediate axial patterning (2). Systemic GDF-11 levels decline with age and administration of higher levels of GDF-11 can reverse age-related cardiac hypertrophy (3). In addition, systemic administration of recombinant GDF-11 protein restores genomic integrity and health of muscle stem cells, neurovasculature and enhances neurogenesis (4, 5).
  • References:
    1. Nakashima, M. et al. (1999) Mech. Dev. 80:185.  
    2. Oh, S.P. et al. (2002) Genes & Dev. 16:274.
    3. Loffredo, F.S. et. al. (2013) Cell. 153.828.
    4. Katsimpardi, L. et. al. (2014) Science. 344.630.
    5. Sinha, M. et. al. (2014) Science. 344.649.
  • Long Name:
    Growth Differentiation Factor 11/8
  • Entrez Gene IDs:
    10220 (Human)
  • Alternate Names:
    GDF-11/GDF-8
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Mouse IgG VisUCyte HRP Polymer Antibody

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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