This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human GDF‑11/BMP‑11 Monoclonal Antibody (Catalog # MAB19584).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human GDF-11/BMP-11 DuoSet ELISA Kit (Catalog # DY1958-05) for convenient development of a sandwich ELISA.
Measured by its ability to neutralize GDF‑11/BMP‑11 induction of hemoglobin expression in the K562 human chronic myelogenous leukemia cell line, Schwall, R.H. et al. (1991) Method Enzymol. 198:340. The Neutralization Dose (ND50) is typically 0.04-0.2 μg/mL in the presence of 25 ng/mL Recombinant Human GDF‑11/BMP‑11. This antibody also neutralizes GDF-8 in a similar K562 bioassay.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Induction of Hemoglobin Expression by GDF‑11/BMP‑11 and Neutralization by anti-GDF‑11/GDF‑8 Antibody.
Recombinant Human GDF-11/BMP-11 (Catalog # 1958-GD) induces hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the pseudoperoxidase assay. Induction of hemoglobin expression by GDF‑11/BMP‑11 (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human GDF‑11/GDF‑8 Monoclonal Antibody (Catalog # MAB19582). The ND50 is typically 0.04-0.2 μg/mL. This antibody also neutralizes GDF-8 in a similar K562 bioassay.
Human GDF‑11/BMP‑11 ELISA Standard Curve.
Recombinant Human GDF‑11/BMP‑11 protein was serially diluted 2-fold and captured by Mouse Anti-Human GDF‑11/BMP‑11 Monoclonal Antibody (Catalog # MAB19584) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GDF‑11/GDF‑8 Monoclonal Antibody (Catalog # MAB19582) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Growth Differentiation Factor 11 (GDF-11), also known as BMP-11, is a member of the TGF-beta superfamily and is highly related to GDF-8. GDF-11 encodes a 407 amino acid (aa) prepropeptide processed into a 109 aa mature protein. Mature GDF-11 contains the canonical 7-cysteine motif common to other TGF-beta superfamily members; however, like the TGF-beta s, Activins and GDF-8, GDF-11 also contains one extra pair of cysteine residues. At the amino acid sequence level, mature human, mouse, rat and chicken GDF-11 are 99-100% identical. Mature GDF-11 and GDF-8 share 90% amino acid sequence identity. GDF-11 is expressed in diverse regions of the mouse embryo: tailbud, somitic precursors, limbs, mandibular and branchial arches, dorsal neural tube, odontoblasts, nasal epithelium, and particular regions of the brain (1). GDF-11 signals through the Activin type II receptors and induces phosphorylation of Smad2 to mediate axial patterning (2). Systemic GDF-11 levels decline with age and administration of higher levels of GDF-11 can reverse age-related cardiac hypertrophy (3). In addition, systemic administration of recombinant GDF-11 protein restores genomic integrity and health of muscle stem cells, neurovasculature and enhances neurogenesis (4, 5).
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