Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 829812
View all GFER/ALR Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GFER/ALR
Arg82-Asp205
Accession # P55789

Specificity

Detects human GFER/ALR in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human GFER/ALR Antibody

Detection of Human GFER/ALR antibody by Western Blot.

Detection of Human GFER/ALR by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GFER/ALR Monoclonal Antibody (Catalog # MAB7940) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GFER/ALR at approximately 20-24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GFER/ALR antibody in Human Liver by Immunohistochemistry (IHC-P).

GFER/ALR in Human Liver.

GFER/ALR was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human GFER/ALR Monoclonal Antibody (Catalog # MAB7940) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human GFER/ALR Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFER/ALR

GFER, also known as Augmenter of Liver Regeneration (ALR) and Hepatopoietin, is an approximately 30 kDa mitochondrial sulfhydryl oxidase that plays a role in protein disulfide bond formation. Alternative splicing of human GFER generates a 15 kDa secreted isoform that lacks the 80 N-terminal amino acids. Within aa 82‑205, human GFER shares approximately 86% aa sequence identity with mouse and rat GFER. GFER protects hepatocytes from toxin-induced damage, promotes hepatic regeneration, and inhibits the metastasis of hepatocellular carcinoma. It also restricts the proliferation of pluripotent and hematopoietic stem cells and maintains mitochondrial integrity.

Long Name

Growth Factor, Augmenter of Liver Regeneration

Alternate Names

ALR, ERV1, Hepatopoietin, HERV1, HPO

Entrez Gene IDs

2671 (Human); 11692 (Mouse); 27100 (Rat)

Gene Symbol

GFER

UniProt

P55789

Additional GFER/ALR Products

Product Documents for Human GFER/ALR Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GFER/ALR Antibody

For research use only

Related Research Areas

Oxidases and Oxygenases
Redox Enzymes

Citations for Human GFER/ALR Antibody

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Protocols

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