Human GFER/ALR Antibody

Catalog # Availability Size / Price Qty
MAB7940
MAB7940-SP
Detection of Human GFER/ALR by Western Blot.
2 Images
Product Details
Citations (1)
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Human GFER/ALR Antibody Summary

Species Reactivity
Human
Specificity
Detects human GFER/ALR in ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 829812
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GFER/ALR
Arg82-Asp205
Accession # P55789
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human GFER/ALR antibody by Western Blot. View Larger

Detection of Human GFER/ALR by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and Hep3B human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GFER/ALR Monoclonal Antibody (Catalog # MAB7940) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for GFER/ALR at approximately 20-24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry GFER/ALR antibody in Human Liver by Immunohistochemistry (IHC-P). View Larger

GFER/ALR in Human Liver. GFER/ALR was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human GFER/ALR Monoclonal Antibody (Catalog # MAB7940) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFER/ALR

GFER, also known as Augmenter of Liver Regeneration (ALR) and Hepatopoietin, is an approximately 30 kDa mitochondrial sulfhydryl oxidase that plays a role in protein disulfide bond formation. Alternative splicing of human GFER generates a 15 kDa secreted isoform that lacks the 80 N-terminal amino acids. Within aa 82‑205, human GFER shares approximately 86% aa sequence identity with mouse and rat GFER. GFER protects hepatocytes from toxin-induced damage, promotes hepatic regeneration, and inhibits the metastasis of hepatocellular carcinoma. It also restricts the proliferation of pluripotent and hematopoietic stem cells and maintains mitochondrial integrity.

Long Name
Growth Factor, Augmenter of Liver Regeneration
Entrez Gene IDs
2671 (Human); 11692 (Mouse); 27100 (Rat)
Alternate Names
ALR; ALRFAD-linked sulfhydryl oxidase ALR; Augmenter of liver regeneration; EC 1.8.3.2; ERV1 homolog; ERV1; ERV1hepatopoietin protein; erv1-like growth factor; GFER; growth factor, augmenter of liver regeneration; growth factor, erv1 (S. cerevisiae)-like (augmenter of liver regeneration); Hepatopoietin; HERV1; HERV1hepatic regenerative stimulation substance; HPO; HPO1; HPO2; HSS; truncated augmenter of liver regeneration

Product Datasheets

Citation for Human GFER/ALR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. ERV1 Overexpression in Myeloid Cells Protects against High Fat Diet Induced Obesity and Glucose Intolerance
    Authors: C Sima, E Montero, D Nguyen, M Freire, P Norris, CN Serhan, TE Van Dyke
    Sci Rep, 2017;7(1):12848.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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