Human GIT1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
Human GIT1 ELISA Standard Curve.
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Product Details
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Human GIT1 Antibody Summary

Species Reactivity
Detects human GIT1 in direct ELISAs. In sandwich ELISAs, this antibody is specific for human GIT1 when paired with the suggested capture antibody.
Recombinant Monoclonal Mouse IgG2B Clone # 924616R
Protein A or G purified from cell culture supernatant
E. coli-derived recombinant human GIT1
Accession # Q9Y2X7
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human/Mouse/Rat GIT1 Monoclonal Antibody (Catalog # MAB8508). In sandwich ELISAs, this antibody is specific for human GIT1 when paired with the suggested capture antibody.

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human GIT1 DuoSet ELISA Kit (Catalog # DY8485-05) for convenient development of a sandwich ELISA.


Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Human GIT1 ELISA Standard Curve. Recombinant Human GIT1 protein was serially diluted 2-fold and captured by Mouse Anti-Human/Mouse/Rat GIT1 Monoclonal Antibody (Catalog # MAB8508) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human GIT1 Monoclonal Antibody (Catalog # MAB85081) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GIT1

GIT1 (ARF GTPase-activating protein GIT1) is a 95 kDa protein that belongs to ADP ribosylation factor family and is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and cell migration. G-protein coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin. GIT1 has also been implicated in neuronal functions including synapse formation and the pathology of Huntington disease. Huntington disease is a genetic neurodegenerative condition involving a mutation in the huntington gene. The huntington gene product (htt) is ubiquitinated and degraded in human Huntington disease brains. Htt interacts directly with GIT1 causing enhanced htt proteolysis, indicating that GIT1 distribution and function may contribute to Huntington disease pathology. Within amino acids (aa) 485-636, human and mouse GIT1 share 93% aa sequence identity.

Long Name
G Protein-Coupled Receptor Kinase Interacting ArfGAP 1
Entrez Gene IDs
28964 (Human); 216963 (Mouse); 83709 (Rat)
Alternate Names
ARF GAP GIT1; ARF GTPase-activating protein GIT1; CAT1; CAT-1; Cool-associated and tyrosine-phosphorylated protein 1; G protein-coupled receptor kinase interacting ArfGAP 1; G protein-coupled receptor kinase interactor 1; G protein-coupled receptor kinase-interactor 1; GIT1; GRK-interacting protein 1

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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