Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 727425
View all GKAP/DLGAP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GKAP
Lys368-Glu472
Accession # O14490

Specificity

Detects human GKAP in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GKAP/DLGAP1 Antibody

GKAP antibody in Human Brain by Immunohistochemistry (IHC-P).

GKAP in Human Brain.

GKAP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human GKAP Monoclonal Antibody (Catalog # MAB7296) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human GKAP/DLGAP1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cortex)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GKAP/DLGAP1

GKAP, also known as DAP1, DLGAP, and SAPAP1, is an approximately 45 kDa cytoplasmic membrane associated protein. GKAP is a component of a neuronal postsynaptic complex that also includes PSD-95 and SHANK. This complex links neurotransmitter channels with the cytoskeleton and can promote NMDA R channel opening. Alternate splice forms of human GKAP have N-terminal and/or C-terminal truncations and are differentially expressed during brain development. Within aa 368-472 human, mouse, and rat GKAP share 100% aa sequence identity.

Long Name

Guanylate Kinase-associated Protein

Alternate Names

DAP1, DLGAP1, SAPAP1

Entrez Gene IDs

9229 (Human); 224997 (Mouse); 65040 (Rat)

Gene Symbol

DLGAP1

UniProt

O14490 (Human)

Additional GKAP/DLGAP1 Products

Product Documents for Human GKAP/DLGAP1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GKAP/DLGAP1 Antibody

For research use only

Citations for Human GKAP/DLGAP1 Antibody

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Protocols

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