Human GLP-1R Antibody

Catalog # Availability Size / Price Qty
MAB28141
MAB28141-SP
GLP‑1R in Human Brain.
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Product Details
Citations (2)
FAQs
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Human GLP-1R Antibody Summary

Species Reactivity
Human
Specificity
Detects human GLP‑1R. Stains human GLP-1R-transfected Baf/3 cells but not irrelevant transfectants.
Source
Monoclonal Mouse IgG2A Clone # 419208
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Baf/3 cell line transfected with human GLP-1R
Accession # P43220
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry GLP‑1R in Human Brain. View Larger

GLP‑1R in Human Brain.
GLP‑1R was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using 3 µg/mL Mouse Anti-Human GLP‑1R Monoclonal Antibody (Catalog # MAB28141) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLP-1R

Glucagon-Like Peptide-1 Receptor (GLP-1R) is a class B G protein-linked seven transmembrane domain protein that is expressed in pancreas, lung, heart, kidney, hypothalamus, and stomach. GLP-1R functions as a receptor for glucagon-like peptide 1 and regulates insulin and glucagon production.

Long Name
Glucagon-like Peptide 1 Receptor
Entrez Gene IDs
2740 (Human); 14652 (Mouse)
Alternate Names
GLP-1 receptor; GLP1R; GLP-1R; GLP-1-R; glucagon-like peptide 1 receptor; MGC138331

Product Datasheets

Citations for Human GLP-1R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Nutrient induced type 2 and chemical induced type 1 experimental diabetes differently modulate gastric GLP-1 receptor expression.
    Authors: Bloch, Olga, Broide, Efrat, Ben-Yehudah, Gilad, Cantrell, Dror, Shirin, Haim, Rapoport, Micha J
    J Diabetes Res, 2015;2015(0):561353.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  2. A novel humanized GLP-1 receptor model enables both affinity purification and Cre-LoxP deletion of the receptor.
    Authors: Jun, Lucy S, Showalter, Aaron D, Ali, Nosher, Dai, Feihan, Ma, Wenzhen, Coskun, Tamer, Ficorilli, James V, Wheeler, Michael, Michael, M Dodson, Sloop, Kyle W
    PLoS ONE, 2014;9(4):e93746.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC - Not specified

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

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Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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