Human GLP-2R Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human GLP‑2R. Stains human GLP-2R transfectants but not irrelevant transfectants.
  • Source
    Monoclonal Mouse IgG2A Clone # 413801
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    HEK293 human embryonic kidney cell line transfected with human GLP-2R
    Met1-Ile553
    Accession # O95838
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunocytochemistry
    8-25 µg/mL
    Immersion fixed HeLa human cervical epithelial carcinoma cell line
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of GLP‑2R in NS0 Mouse Cell Line Transfected with Human GLP-2R by Flow Cytometry. NS0 mouse myeloma cell line transfected with human GLP-2R was stained with Mouse Anti-Human GLP‑2R Monoclonal Antibody (Catalog # MAB4285, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GLP-2R

GLP-2R is a member of the class II glucagon-secretin G protein-coupled receptor superfamily. It is expressed on enteroendocrine cells in the gut where it mediates the positive effects of GLP2 on villus height, crypt depth and intestinal energy absorbtion. After stimulating a cAMP response, lipid raft-dependent internalization of GLP‑2R results in rapid desensitization to GLP2. Extracellular portions of human GLP-2R show approximately 80% amino acid identity with corresponding regions of mouse GLP-2R.

  • Long Name:
    Glucagon-like Peptide 2 Receptor
  • Entrez Gene IDs:
    9340 (Human); 93896 (Mouse); 60432 (Rat)
  • Alternate Names:
    GLP-2 receptor; GLP2R; GLP-2R; GLP-2-R; glucagon-like peptide 2 receptor
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