Human Glypican 6 Antibody

Catalog # Availability Size / Price Qty
AF2845
AF2845-SP
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Human Glypican 6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Glypican 6 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Glypican 6
Asp24-Val527
Accession # Q9Y625
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Glypican 6 (Catalog # 2845-GP)
Flow Cytometry
2.5 µg/106 cells
HepG2 human hepatocellular carcinoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 6

The Glypicans (glypiated proteoglycans) are a small multigene family of GPI-linked heparan sulfate (HS) proteoglycans that likely play a key role in embryonic morphogenesis (1, 2, 3, 4). There are currently six known mammalian Glypicans. They all share a common-sized protein core of 60‑70 kDa, an N-terminus which likely forms a compact globular domain, 14 conserved cysteines that form multiple intrachain disulfide bonds, and a number of C-terminal N- and O-linked carbohydrate attachment sites. Based on exon organization and the location of O-linked glycosylation sites, at least two subfamilies of Glypicans are known, with one subfamily containing Glypicans 1, 2, 4 and 6, and another subfamily containing Glypicans 3 and 5 (3, 5). Human Glypican 6 (GPC-6) is synthesized as a 554 amino acid (aa) preproprecursor that contains a 23 aa signal sequence, a 505 aa mature region and a 26 aa C-terminal prosegment (5, 6). There are four consecutive Ser-Gly repeats that serve as a heparin sulfate attachment site. GPC-6 is reported to be as large as 110 kDa in size. This translates into approximately 50 kDa of proteoglycan (5). Human to mouse, there is 97% aa identity over the entire GPC-6 molecule. Cells known to express GPC-6 are adult ovary and embryonic vascular and visceral smooth muscle, plus mesenchyme (embryonic connective tissue) in multiple organs (1, 5, 6). The function of GPC-6 is essentially unknown. As a Glypican family member, it may facilitate heparin-binding growth factor signaling and polyamine uptake into expressing cells (7, 8). In this regard, it would appear that GPC-6 with its attendant HS is downregulated by triiodothyronine during cartilage maturation, thus limiting the availability of sites for FGF sequestration and activity (9).

References
  1. Song, H.H. and J. Filmus (2002) Biochim. Biophys. Acta 1573:241. 
  2. Filmus, J. (2001) Glycobiology 11:19R. 
  3. De Cat, B. and G. David (2001) Semin. Cell Dev. Biol. 12:117. 
  4. Filmus, J. (2003) Glycoconj. J. 19:319. 
  5. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
  6. Paine-Saunders, S. et al. (1999) Genomics 57:455.
  7. Fransson, L-A. et al. (2004) Cell Mol. Life Sci. 61:1016.
  8. Fransson, L-A. (2003) Int. J. Biochem. Cell Biol. 35:125.
  9. Bassett, J.H.D. et al. (2006) Endocrinology 147:295.
Entrez Gene IDs
10082 (Human); 23888 (Mouse)
Alternate Names
Glypican 6; glypican proteoglycan 6; glypican-6; GPC6; MGC126288; OMIMD1

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Secondary Antibodies

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