Human GM-CSF Antibody

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(2 Reviews)
    
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Citations (6)
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  • Species Reactivity
    Human
  • Specificity
    Detects human GM‑CSF in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse GM‑CSF is observed. Neutralizes the biological activity of both recombinant human GM‑CSF and natural human GM‑CSF.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human GM-CSF
    Ala18-Glu144
    Accession # P04141
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human GM-CSF (Catalog # 215-GM)
  • Neutralization
    Measured by its ability to neutralize GM‑CSF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.08-0.16 µg/mL in the presence of 0.5 ng/mL Recombinant Human GM‑CSF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Cell Proliferation Induced by GM‑CSF and Neutralization by Human GM‑CSF Antibody.

Recombinant Human GM‑CSF (Catalog # 215-GM) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human GM‑CSF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human GM‑CSF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑215‑NA). The ND50 is typically 0.08-0.16 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GM-CSF

GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils (1, 2). GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity (3‑5). It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines (6, 7). The 22 kDa glycosylated GM-CSF, similar to IL-3 and IL-5, is a cytokine with a core of four bundled alpha ‑helices (8‑12). Mature human GM-CSF shares 63%‑70% amino acid sequence identity with canine, feline, porcine, and rat GM-CSF and 54% with mouse GM-CSF. GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF R alpha /CD116 and the signal transducing common beta  chain (CD131) which is also a component of the high-affinity receptors for IL-3 and IL-5 (13, 14). In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF R alpha (15). Human GM-CSF is active on canine and feline cells but not on murine cells (16‑18).

  • References:
    1. Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.
    2. Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.
    3. Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.
    4. Cao, Y. (2007) J. Clin. Invest. 117:2362.
    5. Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.
    6. Heuser, M. et al. (2007) Semin. Hematol. 44:148.
    7. Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.
    8. Kaushansky, K. et al. (1992) Biochemistry 31:1881.
    9. Diederichs, K. et al. (1991) Science 254:1779. 
    10. Cantrell, M.A. et al. (1985) Proc. Natl. Acad. Sci. 82:6250.
    11. Lee, F. et al. (1985) Proc. Natl. Acad. Sci. 82:4360.
    12. Wong, G.G. et al. (1985) Science 228:810.
    13. Onetto-Pothier, N. et al. (1990) Blood 75:59.
    14. Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.
    15. Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.
    16. Hogge, G.S. et al. (1990) Cancer Gene Ther. 6:26.
    17. Sprague, W.S. et al. (2005) J. Comp. Pathol. 133:136.
    18. Shanafelt, A.B. et al. (1991) J. Biol. Chem. 266:13804.
  • Long Name:
    Granulocyte Macrophage Growth Factor
  • Entrez Gene IDs:
    1437 (Human); 12981 (Mouse); 116630 (Rat); 397208 (Porcine); 403923 (Canine); 493805 (Feline)
  • Alternate Names:
    colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF; CSF2; GMCSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897; molgramostin; sargramostim
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Decidual GM-CSF is a critical common intermediate necessary for thrombin and TNF induced in-vitro fetal membrane weakening.
    Authors: Kumar D, Moore R, Nash A, Springel E, Mercer B, Philipson E, Mansour J, Moore J
    Placenta, 2014;35(12):1049-56.
    Species: Human
    Sample Type: Whole Tissue
    Application: Neut
  2. Low concentrations of recombinant granulocyte macrophage-colony stimulating factor derived from Chinese hamster ovary cells augments long-term bioactivity with delayed clearance in vitro.
    Authors: Hashimoto A, Tanaka T, Itoh Y, Yamagata A, Kitamura N, Tazawa R, Nakagaki K, Nakata K
    Cytokine, 2014;68(2):118-26.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Apoptotic neutrophils release macrophage migration inhibitory factor upon stimulation with tumor necrosis factor-alpha.
    Authors: Daryadel A, Grifone RF, Simon HU, Yousefi S
    J. Biol. Chem., 2006;281(37):27653-61.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions.
    Authors: Bartemes KR, Cooper KM, Drain KL, Kita H
    J. Allergy Clin. Immunol., 2005;116(4):827-35.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Toxoplasma gondii induces granulocyte colony-stimulating factor and granulocyte-macrophage colony-stimulating factor secretion by human fibroblasts: implications for neutrophil apoptosis.
    Authors: Channon JY, Miselis KA, Minns LA, Dutta C, Kasper LH
    Infect. Immun., 2002;70(11):6048-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
    Authors: Sato E, Koyama S
    Eur. Respir. J., 2000;16(5):951-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut

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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 203  
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB , Simple Western HAF109 20  
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Goat IgG HRP-conjugated Antibody

WB HAF017 16  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL003 6
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 7  
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB , ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 4 (Based on 2 reviews)

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We have 2 review tested in 1 species: Human.
We have 2 reviews tested in 2 applications: Immunohistochemistry, Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Human GM-CSF Antibody AF-215-NA Array AF-215-NA
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 IHC Human Anonymous 03/27/2018
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Immunohistochemistry Human GM-CSF Antibody AF-215-NA
Immunohistochemistry: Human GM-CSF Antibody [AF-215-NA].

Summary

Application Immunohistochemistry
Sample Tested Adult brain
Species Human

Other Experimental Details

Other Experimental Details Published in https://www.ncbi.nlm.nih.gov/pubmed/28169287Used at 10ug/ml.Briefly, frozen brain sections were fixed in 4% PFA(Fisher Scientific), followed by antigen retrieval using heating in acidcitric buffer (Vector, Burlingame, CA, USA). Endogenous avidin-biotin was blocked for 15 min (Vector).Sections were incubated with 10% horse serum in PBS (Biosera, Boussens, France) and Fc ReceptorBlocking Solution was added (Human TruStain FcX Biolegend,London, UK). Primary antibodies were added overnight at 4 °C.GM-CSF was detected with donkey anti-goat-biotin (ab6578,Abcam), followed by streptavidin-alkaline phosphatase (SA-5100,Vector) and visualised with the Vector Blue Alkaline PhosphataseSubstrate Kit III (Vector).
Western Blot Human GM-CSF Antibody AF-215-NA Array AF-215-NA
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 WB Human Anonymous 07/01/2016
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Western Blot Human GM-CSF Antibody AF-215-NA
Western Blot: Human GM-CSF Antibody [AF-215-NA].

Summary

Application Western Blot
Sample Tested Recombinant GM-CSF
Species Human

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