Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1024936
View all GM2/GD2 Synthase/ B4GALNT1 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GM2/GD2 Synthase/ B4GALNT1
Ser26-Gln533
Accession # Q00973

Specificity

Detects human GM2/GD2 Synthase/ B4GALNT1in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human GM2/GD2 Synthase/ B4GALNT1 Antibody

GM2/GD2 Synthase/ B4GALNT1 in U‑251 MG Human Cell Line.

GM2/GD2 Synthase/ B4GALNT1 was detected in immersion fixed U‑251 MG human glioblastoma cell line using Mouse Anti-Human GM2/GD2 Synthase/ B4GALNT1 Monoclonal Antibody (Catalog # MAB105031) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human GM2/GD2 Synthase/ B4GALNT1 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U‑251 MG human glioblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GM2/GD2 Synthase/ B4GALNT1

B4GALNT1, also known as GM2/GD2 Synthase, is an enzyme involved in the biosynthesis of brain-enriched complex gangliosides GM2, GD2, GT2 and GA2 from GM3, GD3, GT3 and GA3.  This is a critical step in the synthesis of all complex gangliosides enriched in the nervous system of vertebrates.

Long Name

Beta-1,4-N-Acetyl-Galactosaminyltransferase 1

Alternate Names

GALGT, GALNACT, GD2 Synthase, GM2 Synthase, SPG26

Entrez Gene IDs

2583 (Human); 14421 (Mouse)

Gene Symbol

B4GALNT1

UniProt

Q00973

Additional GM2/GD2 Synthase/ B4GALNT1 Products

Product Documents for Human GM2/GD2 Synthase/ B4GALNT1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GM2/GD2 Synthase/ B4GALNT1 Antibody

For research use only

Related Research Areas

Glycobiology-related Enzymes

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