Human GM2/GD2 Synthase/ B4GALNT1 Antibody

Catalog #: MAB105031 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB105031-100
MAB105031-SP

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GM2/GD2 Synthase/ B4GALNT1 in U‑251 MG Human Cell Line.

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Human GM2/GD2 Synthase/ B4GALNT1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GM2/GD2 Synthase/ B4GALNT1in direct ELISAs.

Source

Monoclonal Mouse IgG_2A Clone # 1024936

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GM2/GD2 Synthase/ B4GALNT1
Ser26-Gln533
Accession # Q00973

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

Immersion fixed U‑251 MG human glioblastoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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GM2/GD2 Synthase/ B4GALNT1 in U‑251 MG Human Cell Line. GM2/GD2 Synthase/ B4GALNT1 was detected in immersion fixed U‑251 MG human glioblastoma cell line using Mouse Anti-Human GM2/GD2 Synthase/ B4GALNT1 Monoclonal Antibody (Catalog # MAB105031) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GM2/GD2 Synthase/ B4GALNT1

B4GALNT1, also known as GM2/GD2 Synthase, is an enzyme involved in the biosynthesis of brain-enriched complex gangliosides GM2, GD2, GT2 and GA2 from GM3, GD3, GT3 and GA3. This is a critical step in the synthesis of all complex gangliosides enriched in the nervous system of vertebrates.

Long Name

Beta-1,4-N-Acetyl-Galactosaminyltransferase 1

Entrez Gene IDs

2583 (Human); 14421 (Mouse)

Alternate Names

(N-acetylneuraminyl)-galactosylglucosylceramide; B4GALNT1; beta-1,4 N-acetylgalactosaminyltransferase 1; beta1,4GalNAc-T; beta-1,4-N-acetyl-galactosaminyl transferase 1; beta1-4GalNAc-T; GALGT; GALGTEC 2.4.1.92; GALNACT; GalNAc-T; GD2 Synthase; GD2 synthase, GM2 synthase; GM2 Synthase; GM2/GD2 synthase; SIAT2; SPG26; UDP-Gal:betaGlcNAc beta-1,4-N-acetylgalactosaminyltransferase transferase 1; UDP-N-acetyl-alpha-D-galactosamine:(N-acetylneuraminyl)-galactosylglucosylceramideN-acetylgalactosaminyltransferase (GalNAc-T)