Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Goosecoid Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Goosecoid
Gly48-Arg159
Accession # P56915

Specificity

Detects human Goosecoid in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Goosecoid Antibody

Goosecoid antibody in BG01V Human Embryonic Stem Cells by Immunocytochemistry (ICC).

Goosecoid in BG01V Human Embryonic Stem Cells.

Goosecoid was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Goosecoid Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4086) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Applications for Human Goosecoid Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells differentiated to mesoderm with 100 ng/mL recombinant human EGF Activin A (Catalog # 338-AC-005) for 5 days under low serum conditions

Western Blot

0.1 µg/mL
Sample: Recombinant Human Goosecoid

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Goosecoid

Goosecoid (GSC) is a 28 kDa member of the paired homeobox family. Human Goosecoid is 257 amino acids (aa) in length, and contains one DNA-binding homeodomain (aa 160‑219). Goosecoid appearance correlates with the start of gastrulation and the development of both head/neck and upper body limb mesenchyme. It also appears abnormally in some tumors where it is associated with an epithelial-to-mesenchymal transition. Human Goosecoid shares 97% aa sequence identity with mouse Goosecoid.

Alternate Names

GSC

Entrez Gene IDs

145258 (Human); 14836 (Mouse); 317715 (Rat)

Gene Symbol

GSC

UniProt

P56915

Additional Goosecoid Products

Product Documents for Human Goosecoid Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Goosecoid Antibody

For research use only

Citations for Human Goosecoid Antibody

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Protocols

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