Goosecoid in BG01V Human Embryonic Stem Cells.
Goosecoid was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Goat Anti-Human Goosecoid Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4086) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Goosecoid (GSC) is a 28 kDa member of the paired homeobox family. Human Goosecoid is 257 amino acids (aa) in length, and contains one DNA-binding homeodomain (aa 160‑219). Goosecoid appearance correlates with the start of gastrulation and the development of both head/neck and upper body limb mesenchyme. It also appears abnormally in some tumors where it is associated with an epithelial-to-mesenchymal transition. Human Goosecoid shares 97% aa sequence identity with mouse Goosecoid.
Entrez Gene IDs:
145258 (Human); 14836 (Mouse); 317715 (Rat)
goosecoid homeobox; Goosecoid; GSC; homeobox protein goosecoid
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