Human GPR15 Antibody

(7 citations)   
  • Species Reactivity
    Human
  • Specificity
    Stains human GPR15-transfected cells but not irrelevant transfectants.
  • Source
    Monoclonal Mouse IgG2B Clone # 367902
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human GPR15
    Met1-Leu360
    Accession # P49685
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of GPR15 in HEK293 Human Cell Line Transfected with human GRP15 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GRP15 and eGFP was stained with and either (A) Mouse Anti-Human GPR15 Monoclonal Antibody (Catalog # MAB3654) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GPR15
GPR15, also known as BOB, is a seven-transmembrane G protein-coupled receptor that is expressed in CD4+ T cells and alveolar macrophages. GPR15 functions as a cellular co-receptor for some isolates of HIV-1, HIV-2, and SIV through interactions with several viral envelope proteins. Human GPR15 shares 96%‑100% amino acid sequence identity with chimpanzee, macaque, and rhesus GPR15, and 76% with mouse GPR15.
  • Long Name:
    G Protein-coupled Receptor 15
  • Entrez Gene IDs:
    2838 (Human); 71223 (Mouse)
  • Alternate Names:
    Bob; Brother of Bonzo; G protein-coupled receptor 15; GPR15; G-protein coupled receptor 15; MGC126828; MGC126830
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Trafficking receptor signatures define blood plasmablasts responding to tissue-specific immune challenge
    Authors: Y Seong, NH Lazarus, L Sutherland, A Habtezion, T Abramson, XS He, HB Greenberg, EC Butcher
    JCI Insight, 2017;2(6):e90233.
    Species: Human
    Sample Type: Whole Cells
    Application: Mass Cytometry
  2. Differential effects of ?4?7 and GPR15 on homing of effector and regulatory T cells from patients with UC to the inflamed gut in vivo
    Gut, 2016;65(10):1642-64.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Role and species-specific expression of colon T cell homing receptor GPR15 in colitis.
    Authors: Nguyen L, Pan J, Dinh T, Hadeiba H, O'Hara E, Ebtikar A, Hertweck A, Gokmen M, Lord G, Jenner R, Butcher E, Habtezion A
    Nat Immunol, 2015;16(2):207-13.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Orphan receptor GPR15/BOB is up-regulated in rheumatoid arthritis.
    Authors: Cartwright A, Schmutz C, Askari A, Kuiper J, Middleton J
    Cytokine, 2014;67(2):53-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Role of C-terminal membrane-proximal basic residues in cell surface trafficking of HIV coreceptor GPR15 protein.
    Authors: Okamoto, Yukari, Bernstein, Joshua D, Shikano, Sojin
    J Biol Chem, 2013;288(13):9189-99.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. The role of the alternative coreceptor GPR15 in SIV tropism for human cells.
    Authors: Kiene M, Marzi A, Urbanczyk A, Bertram S, Fisch T, Nehlmeier I, Gnirss K, Karsten C, Palesch D, Munch J, Chiodi F, Pohlmann S, Steffen I
    Virology, 2012;433(1):73-84.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Phosphorylation-dependent C-terminal Binding of 14-3-3 Proteins Promotes Cell Surface Expression of HIV Co-receptor GPR15.
    Authors: Okamoto Y, Shikano S,
    J. Biol. Chem., 2011;286(9):7171-81.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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