Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-reported

Cited:

Flow Cytometry, CyTof

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 367902
View all GPR15 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human GPR15
Met1-Leu360
Accession # P49685

Specificity

Stains human GPR15-transfected cells but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GPR15 Antibody

Detection of GPR15 antibody in HEK293 Human Cell Line Transfected with human GRP15 and eGFP antibody by Flow Cytometry.

Detection of GPR15 in HEK293 Human Cell Line Transfected with human GRP15 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human GRP15 and eGFP was stained with and either (A) Mouse Anti-Human GPR15 Monoclonal Antibody (Catalog # MAB3654) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human GPR15 Antibody

Application
Recommended Usage

CyTOF-reported

Seong, Y. et al. (2017) JCI Insight. 2(6): e90233. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human whole blood CD4+ lymphocytes and HEK293 human embryonic kidney cell line transfected with human GRP15 and eGFP

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPR15

GPR15, also known as BOB, is a seven-transmembrane G protein-coupled receptor that is expressed in CD4+ T cells and alveolar macrophages. GPR15 functions as a cellular co-receptor for some isolates of HIV-1, HIV-2, and SIV through interactions with several viral envelope proteins. Human GPR15 shares 96%‑100% amino acid sequence identity with chimpanzee, macaque, and rhesus GPR15, and 76% with mouse GPR15.

Long Name

G Protein-coupled Receptor 15

Alternate Names

Bob

Entrez Gene IDs

2838 (Human); 71223 (Mouse)

Gene Symbol

GPR15

UniProt

P49685

Additional GPR15 Products

Product Documents for Human GPR15 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human GPR15 Antibody

For research use only

Related Research Areas

Infectious Diseases

Citations for Human GPR15 Antibody

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