Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 475804
View all GRAP2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GRAP2
Met1-Arg330
Accession # O75791

Specificity

Detects endogenous human GRAP2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GRAP2 Antibody

Detection of Human GRAP2 antibody by Western Blot.

Detection of Human GRAP2 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human GRAP2 Monoclonal Antibody (Catalog # MAB4640) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GRAP2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human GRAP2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 2 reviews rated 3.5 using MAB4640 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GRAP2

GRAP2 (GRB2 related adaptor protein 2), a divergent member of the GRB2/Sem5/Drk family, is an adaptor protein involved in leukocyte-specific protein-tyrosine kinase signaing. GRAP2 shares similar structural organization as GRB2 and GRAP with a central SH2 domain flanked by 2 SH3 domains. However, unlike GRB2 and GRAP, the SH2 domain and the C-terminal SH3 domain of GRAP2 contains a unique 120-amino acid glutamine/proline rich sequence. In T lymphocytes, GRAP2 interacts with LAT and SLP-76 to regulate NF-AT (nuclear factor of activated T cells) and form a signaling complex with HPK1 to mediate the JNK signaling pathway. GRAP2 also interacts with M-CSF R and the activated T cell co-stimulatory receptor CD28.

Long Name

GRB2-related Adaptor Protein 2

Alternate Names

GADS, GRB2L, GRBLG, GRBX, Grf40, GRID, GRPL, Mona

Entrez Gene IDs

9402 (Human); 17444 (Mouse); 366962 (Rat)

Gene Symbol

GRAP2

UniProt

O75791

Additional GRAP2 Products

Product Documents for Human GRAP2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GRAP2 Antibody

For research use only

Customer Reviews for Human GRAP2 Antibody (2)

3.5 out of 5
2 Customer Ratings
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  • Human GRAP2 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human GRAP2 Antibody MAB4640
  • Human GRAP2 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/09/2018

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