Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 691816
View all GRK5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human GRK5
Pro463-Ser590
Accession # P34947

Specificity

Detects human GRK5 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) GRK6 is observed and no cross-reactivity with rhGRK4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human GRK5 Antibody

GRK5 antibody in BJAB Human Cell Line by Immunocytochemistry (ICC).

GRK5 in BJAB Human Cell Line.

GRK5 was detected in immersion fixed BJAB human Burkitt's lymphoma cell line using Mouse Anti-Human GRK5 Monoclonal Antibody (Catalog # MAB4539) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human GRK5 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BJAB human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GRK5

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK5 is a member of the GRK4 subfamily, and is expressed in a wide variety of tissues.

Long Name

G protein-coupled Receptor Kinase 5

Alternate Names

GPRK5

Entrez Gene IDs

2869 (Human); 14773 (Mouse); 59075 (Rat)

Gene Symbol

GRK5

UniProt

P34947

Additional GRK5 Products

Product Documents for Human GRK5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GRK5 Antibody

For research use only

Related Research Areas

Intracellular Kinases

Citations for Human GRK5 Antibody

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