Human GSK-3 beta Antibody

Catalog # Availability Size / Price Qty
MAB25061
MAB25061-SP
Human GSK-3 beta  Antibody in Data
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Product Details
Citations (1)
FAQs
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Human GSK-3 beta Antibody Summary

Species Reactivity
Human
Specificity
Detects human GSK-3 beta.
Source
Monoclonal Rat IgG2B Clone # 272535
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human GSK-3 beta
Met1-Thr420
Accession # P49841
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry GSK‑3 beta  in MCF-7 Human Cell Line. View Larger

GSK‑3 beta in MCF-7 Human Cell Line. GSK‑3 beta was detected in immersion fixed MCF-7 human breast cancer cell line using 10 µg/mL Human GSK‑3 beta Monoclonal Antibody (Catalog # MAB25061) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GSK-3 beta

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified asan inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta, inhibited by phosphorylation at S9 by Akt, is involved inenergy metabolism, body pattern formation, and neuronal cell development.

Long Name
Glycogen Synthase Kinase 3 beta
Entrez Gene IDs
2932 (Human); 56637 (Mouse); 84027 (Rat)
Alternate Names
EC 2.7.11; EC 2.7.11.26; glycogen synthase kinase 3 beta; glycogen synthase kinase-3 beta; GSK3 beta; GSK-3 beta; GSK3B; GSK3beta isoform

Product Datasheets

Citation for Human GSK-3 beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Discovery of novel AKT inhibitors with enhanced anti-tumor effects in combination with the MEK inhibitor.
    Authors: Dumble, Melissa, Crouthamel, Ming-Chi, Zhang, Shu-Yun, Schaber, Michael, Levy, Dana, Robell, Kimberly, Liu, Qi, Figueroa, David J, Minthorn, Elisabet, Seefeld, Mark A, Rouse, Meagan B, Rabindran, Sridhar, Heerding, Dirk A, Kumar, Rakesh
    PLoS ONE, 2014;9(6):e100880.
    Species: Human
    Sample Types: Whole Cells
    Applications: ELISA Development

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Rat IgG2B Isotype Control

Reconstitution Buffers

Secondary Antibodies

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