Hyaluronan and Proteoglycan Link Protein 4, (HAPLN4), also known as brain link protein 2, is one of four secreted HAPLN family proteoglycans that bind hyaluronan. HAPLN4 contains one Ig-type C2-set domain and two link domains. It is predominantly expressed in brain and participates in the development of the perineuronal matrix. Human and mouse HAPLN4 share 91% aa sequence identity.
Human HAPLN4 Antibody
R&D Systems | Catalog # MAB4085
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 387915
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HAPLN4
Gln30-Val402 (predicted)
Accession # Q86UW8
Gln30-Val402 (predicted)
Accession # Q86UW8
Specificity
Detects human HAPLN4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human HAPLN1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Applications for Human HAPLN4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human HAPLN4
Sample: Recombinant Human HAPLN4
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: HAPLN4
Long Name
Hyaluronan and Proteoglycan Link Protein 4
Alternate Names
BRAL2, Lpr4
Gene Symbol
HAPLN4
UniProt
Additional HAPLN4 Products
Product Documents for Human HAPLN4 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human HAPLN4 Antibody
For research use only
Related Research Areas
Citations for Human HAPLN4 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars