Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HAPLN4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human HAPLN4
Gln30-Val402
Accession # Q86UW8

Specificity

Detects human HAPLN4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human HAPLN1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human HAPLN4 Antibody

HAPLN4 antibody in Human Brain by Immunohistochemistry (IHC-P).

HAPLN4 in Human Brain.

HAPLN4 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using 3 µg/mL Goat Anti-Human HAPLN4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4085) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human HAPLN4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (caudate nucleus) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human HAPLN4, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human HAPLN4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HAPLN4

HAPLN4 (hyaluronan and proteoglycan link protein 4; also Bral2) is a 40-42 kDa secreted member of the Link module superfamily. It is secreted by select neurons, particularly of the cerebellum, where it participates in ECM stability. Here it may link hyaluronic acid with resident proteoglycans. Mature HAPLN4 is 383 amino acids (aa) in length. It contains one lg-like C2-type domain (aa 46-161) and two link domains (aa 163-365). One potential alternate start site exists 36 aa upstream of the standard start site. Human and mouse HAPLN4 share 91% aa sequence identity.

Long Name

Hyaluronan and Proteoglycan Link Protein 4

Alternate Names

BRAL2, Lpr4

Entrez Gene IDs

404037 (Human); 330790 (Mouse)

Gene Symbol

HAPLN4

UniProt

Q86UW8

Additional HAPLN4 Products

Product Documents for Human HAPLN4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human HAPLN4 Antibody

For research use only

Citations for Human HAPLN4 Antibody

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Protocols

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