Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HAX-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HAX-1
Ser2-Leu261
Accession # O00165

Specificity

Detects human HAX-1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human HAX‑1 Antibody

Detection of Human HAX‑1 antibody by Western Blot.

Detection of Human HAX‑1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAX-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5458) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HAX-1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

HAX-1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

HAX‑1 in HepG2 Human Cell Line.

HAX-1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human HAX-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5458) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human HAX‑1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HAX-1

HAX-1 (HCLS1-associated protein X-1; also HS1-binding protein1) is a 34‑35 kDa intracellular protein with structural similarity to Bcl-2 family proteins. It is ubiquitously expressed, and participates in a variety of cell-specific functions. HAX‑1 blocks cardiomyocyte apoptosis via caspase‑9 inhibition, downregulates conjugated bile acid secretion via BSEP binding, and via PKD2, transmitts integrin signals to the ER. Human HAX-1 is 279 amino acids (aa) in length and contains two Bcl-2 homology regions (aa 37‑56 and 74‑89), a PEST domain (aa 104‑117) and a potential transmembrane segment (aa 261‑279). HS-1 is found mostly in the mitochondria. HAX-1 is also cleaved by caspase-3 at Asp127. Over aa 2‑261, human HAX-1 shares 81% aa identity with mouse HAX-1.

Long Name

HCLS1 associated protein X-1

Alternate Names

HAX1, HCLSBP1, HS1BP1, SCN3

Entrez Gene IDs

10456 (Human); 23897 (Mouse); 291202 (Rat)

Gene Symbol

HAX1

UniProt

O00165 (Human)

Additional HAX-1 Products

Product Documents for Human HAX‑1 Antibody

Certificate of Analysis

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Product Specific Notices for Human HAX‑1 Antibody

For research use only

Citations for Human HAX‑1 Antibody

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Protocols

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