Detection of Human HAX‑1 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAX-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5458) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HAX‑1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
HAX‑1 in HepG2 Human Cell Line.
HAX‑1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human HAX‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5458) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
HAX-1 (HCLS1-associated protein X-1; also HS1-binding protein1) is a 34‑35 kDa intracellular protein with structural similarity to Bcl-2 family proteins. It is ubiquitously expressed, and participates in a variety of cell-specific functions. HAX‑1 blocks cardiomyocyte apoptosis via caspase‑9 inhibition, downregulates conjugated bile acid secretion via BSEP binding, and via PKD2, transmitts integrin signals to the ER. Human HAX-1 is 279 amino acids (aa) in length and contains two Bcl-2 homology regions (aa 37‑56 and 74‑89), a PEST domain (aa 104‑117) and a potential transmembrane segment (aa 261‑279). HS-1 is found mostly in the mitochondria. HAX-1 is also cleaved by caspase-3 at Asp127. Over aa 2‑261, human HAX-1 shares 81% aa identity with mouse HAX-1.
HCLS1 associated protein X-1
Entrez Gene IDs:
10456 (Human); 23897 (Mouse); 291202 (Rat)
FLJ17042; FLJ93803; HAX1; HAX-1; HCLS1 (and PKD2) associated protein; HCLS1 associated protein X-1; HCLS1-associated protein X-1; HCLSBP1; HS1 binding protein; HS1-associating protein X-1; HS1-binding protein 1; HS1BP1; HS1BP1FLJ18492; HSP1BP-1; SCN3
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