Human HAX-1 Antibody

Catalog # Availability Size / Price Qty
AF5458
AF5458-SP
Detection of Human HAX‑1 by Western Blot.
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Product Details
Citations (1)
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Human HAX-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HAX-1 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human HAX-1
Ser2-Leu261
Accession # O00165
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human HAX‑1 antibody by Western Blot. View Larger

Detection of Human HAX‑1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAX-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5458) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HAX-1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry HAX-1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

HAX‑1 in HepG2 Human Cell Line. HAX-1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Goat Anti-Human HAX-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5458) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAX-1

HAX-1 (HCLS1-associated protein X-1; also HS1-binding protein1) is a 34‑35 kDa intracellular protein with structural similarity to Bcl-2 family proteins. It is ubiquitously expressed, and participates in a variety of cell-specific functions. HAX‑1 blocks cardiomyocyte apoptosis via caspase‑9 inhibition, downregulates conjugated bile acid secretion via BSEP binding, and via PKD2, transmitts integrin signals to the ER. Human HAX-1 is 279 amino acids (aa) in length and contains two Bcl-2 homology regions (aa 37‑56 and 74‑89), a PEST domain (aa 104‑117) and a potential transmembrane segment (aa 261‑279). HS-1 is found mostly in the mitochondria. HAX-1 is also cleaved by caspase-3 at Asp127. Over aa 2‑261, human HAX-1 shares 81% aa identity with mouse HAX-1.

Long Name
HCLS1 associated protein X-1
Entrez Gene IDs
10456 (Human); 23897 (Mouse); 291202 (Rat)
Alternate Names
FLJ17042; FLJ93803; HAX1; HAX-1; HCLS1 (and PKD2) associated protein; HCLS1 associated protein X-1; HCLS1-associated protein X-1; HCLSBP1; HS1 binding protein; HS1-associating protein X-1; HS1-binding protein 1; HS1BP1; HS1BP1FLJ18492; HSP1BP-1; SCN3

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Citation for Human HAX-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. PELO negatively regulates HER receptor signalling and metastasis.
    Authors: Pedersen K, Canals F, Prat A, Tabernero J, Arribas J
    Oncogene, 2013-02-25;33(9):1190-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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